Telomerase activity in immortalized endothelial cells undergoing p53-mediated apoptosis.
Telomerase activity in immortalized endothelial cells undergoing p53-mediated apoptosis.
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DOI：
10.1006/bbrc.1997.7797
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发表时间：
1997-12
影响因子：
3.1
通讯作者：
S. Maxwell;D. Capp;S. A. Acosta
中科院分区：
文献类型：
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作者：
S. Maxwell;D. Capp;S. A. Acosta
许多癌细胞和永生细胞表现出稳定端粒长度的端粒酶活性，并可能参与细胞永生和致癌作用。在造血细胞、黑色素瘤细胞、神经胶质瘤细胞和髓细胞白血病细胞的分化过程中，已经报道了端粒酶的下调。此外，已报道由p53突变体永生化的正常人乳腺上皮细胞表现出端粒酶活化。然而，没有关于端粒酶活性在p53介导的永生化细胞凋亡过程中的信息。我们研究了端粒酶活性在p53诱导的永生化内皮细胞系ECV-304凋亡。ECV-304细胞通过感染促进高水平野生型p53表达的重组腺病毒诱导凋亡。端粒酶活性测定采用端粒重复序列扩增法（TRAP）。端粒酶活性被认为是不受p53的过度表达和永生化内皮细胞凋亡。
Many cancer and immortal cells exhibit telomerase activity that stabilizes telomere lengths and may be involved in cell immortality and carcinogenesis. Downregulation of telomerase has been reported during differentiation of hematopoietic, melanoma, glioma, and myelocytic leukemia cells. Moreover, normal human mammary epithelial cells immortalized by a p53 mutant have been reported to exhibit activation of telomerase. However, no information is available about the activity of telomerase during p53-mediated apoptosis of immortalized cells. We investigated the activity of telomerase during p53-induced apoptosis of the immortalized endothelial cell line ECV-304. ECV-304 cells were induced into apoptosis by infection with a recombinant adenovirus that facilitated expression of high levels of wild-type p53. Telomerase activity was measured by a PCR-based telomeric repeat amplification protocol (TRAP). Telomerase activity was found to be unaffected by overexpression of p53 and apoptosis in immortalized endothelial cells.