Anti-hyperglycaemic effect of labdane diterpenes isolated from the rhizome of Amomum maximum Roxb., an edible plant in Southwest China
Anti-hyperglycaemic effect of labdane diterpenes isolated from the rhizome of Amomum maximum Roxb., an edible plant in Southwest China
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DOI：
10.1080/14786419.2021.1903006
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发表时间：
2021-03-14
影响因子：
2.2
通讯作者：
Chen, Jun-Jia
中科院分区：
文献类型：
--
作者：
Lu, Chuan-Li;Wang, Li-Ning;Chen, Jun-Jia
大豆蔻根茎是一种主要用于南亚和东南亚的民间药物。本研究观察了黄芪乙醇提取物的降血糖作用。在体外α-葡萄糖苷酶测定和链脲佐菌素（STZ）诱导的小鼠餐后高血糖中均显示出最大根茎。此外，以α-葡萄糖苷酶抑制活性为指标，从其乙酸乙酯亚组分中分离得到6个唇形科二萜化合物：amoxanthin A（1）、ottensinin（2）、coronarin D（3）、coronarin D甲醚（4）、isocoronarin D（5）和zerumin（6）。在这些化合物中，2和6对α-葡萄糖苷酶以及STZ诱导的小鼠高餐后血糖水平表现出显著的抑制作用。此外，分子对接分析表明，2和6可以牢固地结合到α-葡萄糖苷酶的活性位点。这些结果表明，化合物2和6是存在于A.最大，这可能表明在糖尿病管理中的潜在有益作用。
Amomum maximum Roxb. rhizome is a fork medicine mainly used in South and Southeast Asia. In present study, the hypoglycaemic effects of the ethanolic extract of A. maximum rhizome were demonstrated both on alpha-glucosidase assay in vitro and streptozotocin (STZ)-induced postprandial hyperglycaemia in mice. Furthermore, six labdane diterpenes, amoxanthin A (1), ottensinin (2), coronarin D (3), coronarin D methyl ether (4), isocoronarin D (5), and zerumin (6), were isolated from its ethyl acetate sub-fraction with the guidance of alpha-glucosidase inhibitory activity. Among these compounds, 2 and 6 exhibited significant inhibitory effect on alpha-glucosidase, as well as on STZ-induced high postprandial blood glucose levels in mice. Additionally, molecular docking analysis revealed that 2 and 6 could firmly bind to the active sites of alpha-glucosidase. These results suggest that compounds 2 and 6 are the main anti-hyperglycaemic agents present in A. maximum, which may demonstrate potential beneficial effects in diabetes management.