Genetic characterization of the Drosophila homologue of coronin
Genetic characterization of the Drosophila homologue of coronin
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DOI：
10.1242/jcs.01034
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发表时间：
2004-04-15
影响因子：
4
通讯作者：
Shashidhara, LS
中科院分区：
文献类型：
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作者：
Bharathi, V;Pallavi, SK;Shashidhara, LS
我们报道了CORO的克隆和特性，它编码果蝇F-肌动蛋白结合蛋白CORO的同源物。CORO的活性等位基因在腿、翅膀和眼睛的发育中产生各种表型，这些表型类似于与肌动蛋白细胞骨架和/或膜运输相关的基因突变所观察到的表型。CORO中的纯合子致命性突变会导致翅膀成像盘中肌动蛋白细胞骨架的破坏。在上皮细胞中，基底外侧隔状连接和根尖粘连连接的形成也受到不利影响。CORO的活性等位基因和致死等位基因都与膜运输所需的基因Synaxin1A存在遗传上的相互作用。它们对DPP及其受体厚静脉的过度表达也表现出增强的反应。使用DPP：：GFP融合结构追踪DPP形态原表明内吞途径存在缺陷，导致DPP沿AP轴均匀分布，而不是从AP边界开始梯度分布。我们的结果提供了涉及F-肌动蛋白包裹的囊泡的内吞/吐出事件和形态梯度的建立之间的遗传联系。
We report cloning and characterization of coro, which codes for the Drosophila homologue of the F-actin binding protein coronin. Viable alleles of coro produce a variety of phenotypes in leg, wing and eye development, which are similar to the phenotypes observed as a result of mutations in genes associated with the actin cytoskeleton and/or membrane trafficking. Homozygous lethal mutations in coro results in the disruption of the actin cytoskeleton in wing imaginal discs. Formation of both basolateral septate junctions and apical adherens junctions are also adversely affected in epithelial cells. Both viable and lethal alleles of coro show genetic interactions with syntaxin1A, a gene required for membrane trafficking. They also show enhanced response to over-expression of Decapentaplegic (Dpp) and its receptor Thick vein. Tracing of Dpp morphogen using a Dpp::GFP fusion construct suggested defects in the endocytic pathway, which resulted in uniform distribution of Dpp along the AP axis rather than a gradient from the AP boundary. Our results provide a genetic link between endocytosis/exocytosis events involving F actin-coated vesicles and the establishment of morphogen gradient.