Regional analysis of neurofilament protein immunoreactivity in the hamster's cortex
Regional analysis of neurofilament protein immunoreactivity in the hamster's cortex
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DOI：
10.1016/j.jchemneu.2005.01.003
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发表时间：
2005-05-01
影响因子：
2.8
通讯作者：
Ptito, M
中科院分区：
文献类型：
--
作者：
Boire, D;Desgent, S;Ptito, M
含有神经丝蛋白的神经元的几个不同群体的层状分布已被用作仓鼠中皮质区的划分标准。SMI-32是一种单克隆抗体，可识别神经丝蛋白中分子量和高分子量亚基上的非磷酸化表位。在食肉动物和灵长类动物中，SMI-32免疫反应在仓鼠新皮质中存在于细胞体，近端树突和轴突的一些中型和大型锥体神经元位于皮质层III，V和VI。在第IV层中也发现了少量标记的多极细胞。神经丝蛋白免疫反应神经元被发现在整个等皮质区。在辅助运动区、岛叶皮质、视皮层内侧部和顶叶联合皮质中，标记细胞很少。我们的数据表明，SMI-32免疫反应细胞可以有效地用于追踪仓鼠大脑中新皮层区域之间的边界。仓鼠皮质中SMI-32免疫反应性的区域分布与由其细胞结构和骨髓结构定义的皮质区相当接近。初级感觉皮质区含有最强烈的SMI-32免疫反应性，也是有髓轴突密度最高的区域。在眼眶、岛叶、嗅周、扣带回和边缘下皮质中发现非常低的SMI-32免疫反应性，这些皮质在有髓轴突中也很差。这支持SMI-32免疫反应性和髓鞘含量之间的关联。(c)2005 Elsevier B. V.保留所有权利。
The laminar distribution of several distinct populations of neurofilament protein containing neurons has been used as a criterion for the delineation of cortical areas in hamsters. SMI-32 is a monoclonal antibody that recognizes a non-phosphorylated epitope on the medium- and high-molecular weight subunits of neurofilament proteins. As in carnivores and primates, SMI-32 immunoreactivity in the hamster neocortex was present in cell bodies, proximal dendrites and axons of some medium and large pyramidal neurons located in cortical layers III, V and VI. A small population of labeled multipolar cells was also found in layer IV. Neurofilament protein immunoreactive neurons were found throughout isocortical areas. Very few labeled cells were encountered in supplemental motor area, insular cortex, medial portion of associative visual cortex and in parietal association cortex. Our data indicate that SMI-32 immunoreactive cells can be efficiently used to trace boundaries between neocortical areas in the hamster's brain. The regional distribution SMI-32 immunoreactivity in the hamster cortex corresponds quite closely with cortical areas as defined by their cytoarchitecture and myeloarchitecture. The primary sensory cortical areas contain the most intense of SMI-32 immunoreactivity and are also those with the highest density of myelinated axons. Very low SMI-32 immunoreactivity was found in orbital, insular, perirhinal, cingulate and infralimbic cortices, which are also poor in myelinated axons. This supports the association between SMI-32 immunoreactivity and myelin contents. (c) 2005 Elsevier B.V. All rights reserved.