Infection of cynomolgus macaques with a recombinant monkeypox virus encoding green fluorescent protein
Infection of cynomolgus macaques with a recombinant monkeypox virus encoding green fluorescent protein
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DOI：
10.1007/s00705-011-1065-1
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发表时间：
2011-10-01
影响因子：
2.7
通讯作者：
Paragas, Jason
中科院分区：
文献类型：
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作者：
Goff, Arthur;Mucker, Eric;Paragas, Jason
猴痘病毒（MPXV）在人类中引起类似天花的水泡脓疱性皮疹疾病，并在非人灵长类动物中产生类似的疾病。为了提高研究人员研究实验性MPXV感染的能力，我们将编码绿色荧光蛋白（GFP）的基因插入猴痘病毒扎伊尔-79中。野生型和MPXV-GFP在细胞培养物中以相似的动力学复制，并且当静脉内注射到食蟹猴中时引起相似的疾病。在MPXV-GFP感染的动物中，荧光灯下的检查有助于在疾病发展过程中识别皮肤病变，并在尸检时识别复制的内部部位。MPXV-GFP可提高抗病毒治疗和疫苗效果的定量评估。
Monkeypox virus (MPXV) causes a vesiculopustular rash illness resembling smallpox in humans and produces a similar disease in nonhuman primates. To enhance the ability of researchers to study experimental MPXV infections, we inserted a gene encoding green fluorescent protein (GFP) into Monkeypox virus Zaire-79. Wild-type and MPXV-GFP replicated with similar kinetics in cell culture and caused a similar disease when injected intravenously into cynomolgus macaques. In MPXV-GFP-infected animals, examination under fluorescent light facilitated the identification of skin lesions during disease development and internal sites of replication at necropsy. MPXV-GFP could improve the quantitative assessment of antiviral therapy and vaccine efficacy.