Differing antibacterial and antibiofilm properties of Polypodium vulgare L. Rhizome aqueous extract and one of its purified active ingredients-osladin
Differing antibacterial and antibiofilm properties of Polypodium vulgare L. Rhizome aqueous extract and one of its purified active ingredients-osladin
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DOI：
10.1016/j.hermed.2019.100261
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发表时间：
2019-09-01
影响因子：
2.3
通讯作者：
Wojnicz, Dorota
中科院分区：
文献类型：
--
作者：
Glensk, Michal;Tichaczek-Goska, Dorota;Wojnicz, Dorota
水龙骨（水龙骨科），常见的多足类，在波兰传统医学中用作祛痰剂，治疗咳嗽，慢性肾炎和肾盂肾炎。这是第一项测试欧洲石蒜根茎水提取物中所含的奥斯拉丁的抗微生物特性的研究。水提液经树脂柱分离、结晶和制备型高效液相色谱分离，得到水提液中的奥斯拉德。高分辨率质谱法用于确认奥斯拉丁的身份。采用时间-杀菌动力学法和生物膜法研究了提取物和纯化的奥斯拉德的抑菌活性和生物膜活性。这些试验是对人类最常见的致尿路感染大肠埃希菌菌株进行的。黄芪水相提取物及其纯化产物对尿路致病菌的存活和生物膜形成均有抑制作用。
Polypodium vulgare L. (Polypodiaceae), the common polypody, is used in Polish traditional medicine as an expectorant to treat cough, chronic nephritis, and pyelonephritis. This is a first study that tests the antimicrobial properties of the osladin contained in P. vulgare rhizome aqueous extract. The osladin was isolated from the aqueous extract by resin column fractionation, crystallization and preparative high-performance liquid chromatography. High-resolution mass spectrometry was used to confirm the identity of osladin. The bacteriostatic and antibiofilm activity of the extract and of purified osladin were investigated using the time-kill kinetic assay and the biofilm assay. These assays were conducted upon the most commonly uropathogenic Escherichia coli strains for humans. P. vulgare rhizome aqueous-phase extract and purified osladin from that extract showed inhibitory effects on the survival and biofilm formation of the uropathogenic bacteria.