Porphyromonas gingivalis induces its uptake by human macrophages and promotes foam cell formation in vitro
Porphyromonas gingivalis induces its uptake by human macrophages and promotes foam cell formation in vitro
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DOI：
10.1016/j.femsle.2004.10.009
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发表时间：
2004-12-01
影响因子：
2.1
通讯作者：
Lalla, E
中科院分区：
文献类型：
--
作者：
Giacona, MB;Papapanou, PN;Lalla, E
牙龈卟啉单胞菌是人类牙周病的病原体，与动脉粥样硬化相关事件的风险增加有关。在这项研究中，我们研究了牙龈卟啉单胞菌感染对人类巨噬细胞泡沫细胞形成的影响，早期动脉粥样硬化的标志，以及牙龈卟啉单胞菌诱导这些细胞摄取的潜力。将人单核细胞衍生的巨噬细胞与低密度脂蛋白一起孵育，并用牙龈卟啉单胞菌FDC 381或其菌毛缺陷突变体DPG 3感染。与菌毛在该过程中的作用一致，与DPG 3相比，菌株381显著增加泡沫细胞形成。在抗生素保护实验中，菌株381的活牙龈卟啉单胞菌的回收率显著高于DPG 3。通过透射电子显微镜，野生型菌株显示出粘附并进入THP-1细胞。这些结果表明，牙龈卟啉单胞菌的特性，使其能够粘附/侵入其他细胞类型也可能是在巨噬细胞中起作用，并在其致动脉粥样硬化的潜力发挥重要作用。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
Porphyromonas gingivalis is an etiologic agent of periodontal disease in humans, which has been linked to an increased risk for atherosclerosis-related events. In this study, we examined the effect of P. gingivalis infection on human macrophages with respect to foam cell formation, the hallmark of early atherogenesis, and the potential of P. gingivalis to induce its uptake by these cells. Human monocyte-derived macrophages were incubated with low density lipoprotein and infected with P. gingivalis FDC381 or its fimbriae deficient mutant, DPG3. Consistent with a role for fimbriae in this process, strain 381 significantly increased foam cell formation as compared to DPG3. Recovery of viable P. gingivalis in antibiotic protection experiments was significantly higher for strain 381 than for DPG3. By transmission electron microscopy, the wild-type strain was shown to adhere to and enter THP-1 cells. These results suggest that properties of P. gingivalis which render it capable of adhering to/invading other cell types may also be operative in macrophages and play an important role in its atherogenic potential. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.