Morphological Indicator for Directed Evolution of Euglena gracilis with a High Heavy Metal Removal Efficiency
Morphological Indicator for Directed Evolution of Euglena gracilis with a High Heavy Metal Removal Efficiency
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具有高重金属去除效率的细小裸藻定向进化的形态指标
DOI：
10.1021/acs.est.0c05278
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发表时间：
2021
影响因子：
11.4
通讯作者：
Goda Keisuke
中科院分区：
文献类型：
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作者：
Xu Muzhen;Harmon Jeffrey;Yuan Dan;Yan Sheng;Lei Cheng;Hiramatsu Kotaro;Zhou Yuqi;Loo Mun Hong;Hasunuma Tomohisa;Isozaki Akihiro;Goda Keisuke
在过去的几十年里，基于微藻的生物修复方法处理重金属（HM）污染的废水，由于其环境友好，成本效益和可持续性引起了广泛的关注。然而，它们的HM去除效率与实际应用相距甚远。定向进化有望有效地开发具有更高HM去除效率的微藻，但没有非侵入性或无标记的指示剂来识别它们。在这里，我们提出了一个智能的细胞形态指标，用于识别HM的去除效率ofEuglena gracilis在一个非侵入性和无标记的方式。具体来说，我们显示了通过我们的机器学习算法识别的形态元特征与19 E的Cu 2+去除效率之间的强单调相关性（斯皮尔曼的ρ =-0.82，P = 2.1 × 10-5）。纤细的克隆。我们的研究结果坚定地表明，形态的E。细薄细胞可以作为一个有效的HM去除效率的指标，因此有很大的潜力，当结合高通量图像激活细胞分选仪，定向进化为基础的发展E。gracilis具有极高的HM去除效率，适用于全球的实际废水处理。
In the past few decades, microalgae-based bioremediation methods for treating heavy metal (HM)-polluted wastewater have attracted much attention by virtue of their environment friendliness, cost efficiency, and sustainability. However, their HM removal efficiency is far from practical use. Directed evolution is expected to be effective for developing microalgae with a much higher HM removal efficiency, but there is no non-invasive or label-free indicator to identify them. Here, we present an intelligent cellular morphological indicator for identifying the HM removal efficiency ofEuglena gracilisin a non-invasive and label-free manner. Specifically, we show a strong monotonic correlation (Spearman’s ρ = −0.82,P= 2.1 × 10–5) between a morphological meta-feature recognized via our machine learning algorithms and the Cu2+removal efficiency of 19E. gracilisclones. Our findings firmly suggest that the morphology ofE. graciliscells can serve as an effective HM removal efficiency indicator and hence have great potential, when combined with a high-throughput image-activated cell sorter, for directed-evolution-based development ofE. graciliswith an extremely high HM removal efficiency for practical wastewater treatment worldwide.