Changes in progenitor populations and ongoing neurogenesis in the regenerating chick spinal cord
Changes in progenitor populations and ongoing neurogenesis in the regenerating chick spinal cord
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DOI：
10.1016/j.ydbio.2009.05.569
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发表时间：
2009-08-15
影响因子：
2.7
通讯作者：
Ferretti, Patrizia
中科院分区：
文献类型：
--
作者：
Whalley, Katherine;Goegel, Stefanie;Ferretti, Patrizia
鸡的脊髓损伤后可以再生，直到高级发育阶段。可以想象的是，干/祖细胞可塑性的变化有助于这种能力的丧失，这发生在E13左右。我们研究了放射状胶质祖细胞的增殖、表型变化和神经发生对脊髓再生的贡献。在E11或E15损伤后，胶质源性放射状胶质细胞标记物没有早期上调。相比之下，在E11，但不是E15损伤后，灰质中的增殖和过渡蛋白表达的上调增加，表明E11脊髓祖细胞群体中的高水平可塑性在后期阶段丢失。随着发育神经祖细胞的变化也得到了E11比E15更高的神经球形成能力的支持。用双皮质素和神经元特异性标记物和BrdU在脊髓切片和分离的细胞中的共标记表明，神经发生在E11小鸡脊髓中是一个持续的过程。这种神经发生似乎在E15时完成。我们的研究结果表明，再生能力的鸡脊髓是不太成熟，更塑料比以前认为，这可能有助于其有利的反应损伤，并建议在维持再生能力的神经发生的作用。(C)2009 Elsevier Inc. All rights reserved.
The chick spinal cord can regenerate following injury until advanced developmental stages. It is conceivable that changes in stem/progenitor cell plasticity contribute to the loss of this capacity, which occurs around E13. We investigated the contribution of proliferation, phenotypic changes in radial glia progenitors, and neurogenesis to spinal cord regeneration. There was no early up-regulation of markers of gliogenic radial glia after injury either at E11 or E15. In contrast, increased proliferation in the grey matter and up-regulation of transitin expression following injury at E11, but not E15, suggested high levels of plasticity within the E11 spinal cord progenitor population that are lost by later stages. Changes in neural progenitors with development were also supported by a higher neurosphere forming ability at E11 than at E15. Co-labelling with doublecortin and neuron-specific markers and BrdU in spinal cord sections and dissociated cells showed that neurogenesis is an ongoing process in E11 chick spinal cords. This neurogenesis appeared to be complete by E15. Our findings demonstrate that the regeneration-competent chick spinal cord is less mature and more plastic than previously believed, which may contribute to its favourable response to injury, and suggest a role for neurogenesis in maintaining regenerative capacity. (C) 2009 Elsevier Inc. All rights reserved.