THE TRAJECTORY OF MITRAL CELL AXONS IN THE RABBIT OLFACTORY CORTEX REVEALED BY INTRACELLULAR HRP INJECTION
THE TRAJECTORY OF MITRAL CELL AXONS IN THE RABBIT OLFACTORY CORTEX REVEALED BY INTRACELLULAR HRP INJECTION
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DOI：
10.1002/cne.902300107
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发表时间：
1984-01-01
影响因子：
2.5
通讯作者：
KISHI, K
中科院分区：
文献类型：
--
作者：
OJIMA, H;MORI, K;KISHI, K
通过用辣根过氧化物酶（HRP）进行细胞内染色，研究了兔二尖瓣细胞的轴突和轴突侧支向嗅觉皮层的投射。染色的二尖瓣细胞轴突从胞体到前梨状皮层（aPC）的最尾端部分进行重建。单个二尖瓣细胞投射到嗅皮层细胞结构上不同的区域，即前嗅核（AON）、aPC和嗅结节（OT）。所有染色的二尖瓣细胞都投射到 SON 和 aPC，大约四分之一的二尖瓣细胞投射到 OT。在 AON 和 aPC 的表面，在侧嗅束 (LOT) 中运行的主轴突以不同的间隔发出数条细小的侧支。每个区域的络脉并不是均匀突出的，而是通常形成斑驳的末端乔木，这些乔木倾向于前后拉长。在 AON 和 aPC 中，每个单个二尖瓣细胞在 Ia 层形成多个末端乔木。支配 OT 的轴突侧支显示出两种类型的投射模式。一种类型的侧枝从嗅球内的主轴突发出，穿过嗅脚的腹内侧部分，而不加入LOT的主要部分，并主要终止于OT的内侧部分。另一种类型的侧支循环从 LOT 中的主轴突出现，向中后方延伸并投射到 OT 的外侧部分。尽管单个二尖瓣细胞投射到嗅觉皮层的几个部分，但它们在每个区域的特定位置形成密集的末端乔木这一事实表明，球皮质连接不是分散的，而是高度选择性的。
The projection of the axon and axon collaterals of mitral cells to the olfactory cortex was studied in the rabbit by intracellular staining with horseradish peroxidase (HRP). The stained mitral cell axons were reconstructed from the soma to the most caudal portion of the anterior piriform cortex (aPC). Single mitral cells projected to cytoarchitectonically different areas of the olfactory cortex, i.e., the anterior olfactory nucleus (AON),the aPC and the olfactory tubercle (OT). All the stained mitral cells projected to both the SON and the aPC, and about one-fourth of the mitral cells projected to the OT. At the surface of the AON and the aPC, the main axon running in the lateral olfactory tract (LOT) gave off several thin collaterals at various intervals. The collaterals did not project evenly in each area but typically formed patchy terminal arbors which tended to be elongated anteroposteriorly. In both the AON and the aPC, each single mitral cell formed several terminal arbors in layer Ia. The axon collaterals innervating the OT showed 2 types of projection patterns. One type of collateral was emitted from the main axon within the olfactory bulb, coursed through the ventromedial portion of the olfactory peduncle without joining the main mass of the LOT and terminated mainly in the medial portion of the OT. The other type of collateral emerged from the main axon in the LOT, coursed medioposteriorly and projected to the lateral portion of the OT. Although individual mitral cells projected to several parts of the olfactory cortex, the fact that they made dense terminal arbors in specific places in each area suggests that the bulbocortical connections are not diffuse but highly selective.