ISOLATION AND IMMUNOCHEMICAL CHARACTERIZATION OF CARBOHYDRATE ANTIGENS PREPARED FROM GROUP-A STREPTOCOCCUS-PYOGENES
ISOLATION AND IMMUNOCHEMICAL CHARACTERIZATION OF CARBOHYDRATE ANTIGENS PREPARED FROM GROUP-A STREPTOCOCCUS-PYOGENES
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DOI：
10.1016/s0174-3031(83)80051-1
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
MCGHEE, JR
中科院分区：
文献类型：
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作者：
HAMADA, S;OKAHASHI, N;MCGHEE, JR
A组糖抗原是从分离的S.化脓性链球菌Sv（A群，M 3型）。细胞壁通过M1内切-N-乙酰胞壁酶的酶促作用溶解。将细胞壁裂解物浓缩并在Sephadex G-100柱上层析。获得了与A组抗血清反应的两个级分。这些由N-乙酰葡糖胺、鼠李糖和肽聚糖组分如谷氨酸、赖氨酸、丙氨酸、N-乙酰胞壁酸和N-乙酰葡糖胺组成。为了比较，用色谱法纯化细胞壁的热三氯乙酸提取物（TCAgA）或全细胞的Rantz和Randall提取物（RRgA）。后2个也含有鼠李糖和葡萄糖胺，并与A组抗血清反应。M1 gA抗原分子量大于TCAgA和RRgA。半抗原抑制研究表明，N-乙酰氨基葡萄糖是最有效的抑制剂。
Group A carbohydrate antigens were prepared from isolated cells walls or whole cells of S. pyogenes strain Sv (group A, M type 3). Cell walls were lysed by the enzymatic action of M1 endo-N-acetylmuramidase. The cell wall lysate was concentrated and chromatographed on a Sephadex G-100 column. Two fractions reactive with group A antisera were obtained. These were composed of N-acetylglucosamine, rhamnose and peptidoglycan components such as glutamic acid, lysine, alanine, N-acetylmuramic acid and N-acetylglucosamine. For comparison, hot trichloroacetic acid extract of the cell walls (TCAgA) or Rantz and Randall extact of the whole cells (RRgA) was purified chromatographically. The latter 2 also contained rhamnose and glucosamine and were reactive with group A antisera. The MW of M1gA antigens were larger than those of TCAgA and RRgA. Hapten inhibition study indicated that N-acetylglucosamine was the most potent inhibitor.