EFFECTS OF ISOFLURANE ON TRANSPORT ACROSS THE BLOOD-BRAIN-BARRIER
EFFECTS OF ISOFLURANE ON TRANSPORT ACROSS THE BLOOD-BRAIN-BARRIER
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DOI：
10.1097/00000542-199203000-00016
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发表时间：
1992-03-01
期刊：
影响因子：
8.8
通讯作者：
WEISS, HR
中科院分区：
文献类型：
--
作者：
CHI, OZ;ANWAR, M;WEISS, HR
血脑屏障（BBB）在调节和限制分子转移到脑间质空间中起重要作用。麻醉剂可能会影响BBB的渗透性。在这项研究中，异氟烷对小的亲水性分子通过血脑屏障的运输的影响进行了研究，通过测量大鼠的血脑转移系数（Ki）和局部脑血流量（rCBF），并通过计算毛细血管通透性表面积（PS）的产品。在对照组中，在异氟烷麻醉下股动脉和静脉插管后，允许大鼠在测量Ki（n = 6）或rCBF（n = 11）之前保持清醒2 h。实验组和对照组分别用1%和2%异氟醚麻醉，经气管插管机械通气。使用C-14-α-氨基异丁酸测量Ki（1% n = 6，2% n = 7），使用C-14-碘安替比林测量rCBF（2% n = 7）。2%异氟烷对13个脑区中的9个脑区的rCBF无影响。与对照组相比，侧部和后部皮质的血流量较少，而髓质和脑桥的血流量较多。1%和2%异氟烷组13个脑区中有11个的Ki值低于对照组。1%和2%异氟烷在任何脑区均无显著差异。所有脑区的Ki平均值为8.9 +/- 4.9 μ l。G-1 .对照组为4.6 ± 2.6和5.1 ± 3.4 μ l。G-1 . min-1，1%和2%异氟醚组差异有显著性（P < 0.0001）。在研究的组中，PS产物与Ki相似。总之，异氟烷麻醉减少了亲水性小分子穿过BBB的转运，Ki和PS积的降低证明了这一点，而rCBF没有发生大的变化。
The blood-brain barrier (BBB) plays an important role in regulating and restricting transfer of molecules into the interstitial space of the brain. Anesthetic agents may affect the permeability of the BBB. In this investigation, the effect of isoflurane on the transport of small hydrophilic molecules across the BBB was studied in rats by measuring the blood-brain transfer coefficient (Ki) and the regional cerebral blood flow (rCBF) and by calculating the capillary permeability-surface area (PS) product. In the control group, after a femoral artery and vein were catheterized under isoflurane anesthesia, rats were allowed to remain awake for 2 h before measuring Ki (n = 6) or rCBF (n = 11). In the two experimental groups, rats were anesthetized with 1% and 2% isoflurane respectively, and their lungs were mechanically ventilated through a tracheal tube. Ki was measured (1% n = 6, 2% n = 7) using C-14-alpha-aminoisobutyric acid, and rCBF was measured (2% n = 7) using C-14-iodoantipyrine. Two percent isoflurane did not affect rCBF in 9 of 13 brain regions. Blood flow was less in the lateral and posterior cortex and greater in the medulla and pons when compared with the control group. Ki was less in 11 of 13 brain regions in both the 1% and 2% isoflurane groups than in the control group. There was no significant difference between 1 and 2% isoflurane in any brain region. The average values of Ki in all of the brain regions were 8.9 +/- 4.9-mu-l . g-1 . min-1 in the control group and 4.6 +/- 2.6 and 5.1 +/- 3.4 mu-l . g-1 . min-1 in the 1% and 2% isoflurane groups, respectively (P < 0.0001). The PS product was similar to Ki in the groups studied. In conclusion, isoflurane anesthesia decreased the transport of small hydrophilic molecules across the BBB, as was demonstrated by decreases in Ki and the PS product without large changes in rCBF.