ROLE OF PROSTAGLANDIN-D2 IN THE HYPOTHERMIA OF RATS CAUSED BY BACTERIAL LIPOPOLYSACCHARIDE
ROLE OF PROSTAGLANDIN-D2 IN THE HYPOTHERMIA OF RATS CAUSED BY BACTERIAL LIPOPOLYSACCHARIDE
复制标题
DOI：
10.1073/pnas.79.19.6093
复制
发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
HAYAISHI, O
中科院分区：
文献类型：
--
作者：
UENO, R;NARUMIYA, S;HAYAISHI, O
腹膜内施用来自鼠伤寒沙门氏菌的脂多糖（1 mg/kg）导致大鼠结肠温度下降约3.5 ℃。2.degree.在环境温度为22 ± 0 ℃时，3.degree. C.吲哚美辛可剂量依赖性地减轻脂多糖引起的体温降低。其他前列腺素合成酶抑制剂如阿司匹林、氟灭酸和保泰松的作用与吲哚美辛相似。当脑室内注射各种前列腺素时，只有前列腺素D2在1.2和6 nmol/kg之间的剂量下引起结肠温度的剂量依赖性下降。将前列腺素D2微量注射到视前区中引起体温降低. apprx。1.degree. C.下丘脑后部注射前列腺素D2对结肠温度影响不大。前列腺素D2引起的体温降低并不因吲哚美辛的给药而减轻。腹腔注射脂多糖1 h后，大鼠脑视前区/下丘脑区的前列腺素D2的量显著增加，而在用吲哚美辛预处理的大鼠中未观察到这种增加。视前区/下丘脑切片与脂多糖的体外孵育也增加了前列腺素D2的量。脂多糖的腹膜内给药诱导大鼠脑的视前区/下丘脑区域中前列腺素D2的释放，并且后一种化合物参与大鼠对脂多糖的低温反应。
The i.p. administration of lipopolysaccharide from Salmonella typhimurium (1 mg/kg) caused a fall in the rat colonic temperature of .apprx. 2.degree. C at an ambient temperature of 22 .+-. 3.degree. C. The hypothermia induced by the lipopolysaccharide was abated in a dose-dependent manner by the administration of indomethacin. Other inhibitors of prostaglandin synthetase such as aspirin, flufenamic acid and phenylbutazone had effects similar to those of indomethacin. When various prostaglandins were injected intracerebroventricularly, only prostaglandin D2 caused a dose-dependent fall in the colonic temperature at doses between 1.2 and 6 nmol/kg. Microinjection of prostaglandin D2 into the preoptic area caused hypothermia of .apprx. 1.degree. C. Injection of prostaglandin D2 into the posterior hypothalamus had little effect on the colonic temperature. The hypothermia caused by prostaglandin D2 was not abated by the administration of indomethacin. The amount of prostaglandin D2 increased significantly in the preoptic/hypothalamic region of rat brain 1 h after the i.p. administration of the lipopolysaccharide, whereas such increase was not observed in rats pretreated with indomethacin. The in vitro incubation of the preoptic/hypothalamic slices with the lipopolysaccharide also increased the amount of prostaglandin D2. The i.p. administration of the lipopolysaccharide induces the release of prostaglandin D2 in the preoptic/hypothalamic area of rat brain and the latter compound is involved in the hypothermic response of rats to the lipopolysaccharide.