Biphasic mini‐reactor for characterization of biocatalyst performance
Biphasic mini‐reactor for characterization of biocatalyst performance
复制标题
用于表征生物催化剂性能的双相微型反应器
DOI：
10.1002/biot.200800271
复制
发表时间：
2009
影响因子：
4.7
通讯作者：
L. Greiner
中科院分区：
文献类型：
--
作者：
Anne van den Wittenboer;T. Schmidt;P. Müller;M. Ansorge‐Schumacher;L. Greiner
双相反应介质正在扩大工业生物催化的范围。在这些条件下影响催化剂性能的因素的彻底调查是催化过程的成功实施的关键。在这里，我们提出了一个反应器设置适合于全面的系统表征和优化的生物催化反应的两相系统具有不同的阶段。它的特点是体积小，允许用最少量的溶剂和催化剂进行可重复的实验。界面面积保持恒定，并允许两相独立搅拌，以最大限度地减少叠加效应。低挥发性有机溶剂的蒸发通过使用气密结构来防止。该微型反应器在间歇和连续模式下测定传质、酶生产率和酶稳定性方面具有广泛的适用性。
Biphasic reaction media are extending the scope of technical biocatalysis. Thorough investigation of the factors affecting catalyst performance under these conditions is of key importance for the successful implementation of catalytic processes. Here, we present a reactor setup suitable for comprehensive systematic characterization and optimization of biocatalyzed reactions in biphasic systems with distinct phases. It is distinguished by small volumes allowing reproducible experimentation with minimum amounts of solvent and catalyst. The interfacial area is kept constant and independent stirring of both phases is allowed in order to minimize superimposing effects. Evaporation of low‐volatile organic solvents is prevented by use of airtight construction. The broad applicability of this mini‐reactor is demonstrated with regard to determination of mass transfer, enzyme productivity, and enzyme stability in both batch and continuous mode.