Enhanced translation by Nucleolin via G-rich elements in coding and non-coding regions of target mRNAs.
Enhanced translation by Nucleolin via G-rich elements in coding and non-coding regions of target mRNAs.
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DOI：
10.1093/nar/gkr488
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发表时间：
2011-10
影响因子：
14.9
通讯作者：
Gorospe M
中科院分区：
文献类型：
--
作者：
Abdelmohsen K;Tominaga K;Lee EK;Srikantan S;Kang MJ;Kim MM;Selimyan R;Martindale JL;Yang X;Carrier F;Zhan M;Becker KG;Gorospe M
RNA结合蛋白（RBP）在许多转录水平上调节基因表达，包括mRNA稳定性和转化，具有四个RNA识别基序的RBP核素已在细胞增殖，癌变和病毒感染中浸渍核靶mRNA及其对其表达的影响尚未在整个转录组水平上进行研究，我们在全球识别出核靶转录本，其中许多编码了与细胞生长和癌症相关的蛋白质核素靶标的签名基序令人惊讶地在3'-非翻译区（UTR）中发现了G型，而且在编码区域（CR）和5'-UTR中也发现了增强了带有G富基序的mRNA的翻译，因为沉默的核素没有改变目标mRNA稳定性，而是降低了在靶转录物上形成的多聚体的大小，并降低了编码蛋白的抽象。在靶mRNA的CR和UTR中，其中许多编码癌症并增强其翻译。
RNA-binding proteins (RBPs) regulate gene expression at many post-transcriptional levels, including mRNA stability and translation. The RBP nucleolin, with four RNA-recognition motifs, has been implicated in cell proliferation, carcinogenesis and viral infection. However, the subset of nucleolin target mRNAs and the influence of nucleolin on their expression had not been studied at a transcriptome-wide level. Here, we globally identified nucleolin target transcripts, many of which encoded cell growth- and cancer-related proteins, and used them to find a signature motif on nucleolin target mRNAs. Surprisingly, this motif was very rich in G residues and was not only found in the 3′-untranslated region (UTR), but also in the coding region (CR) and 5′-UTR. Nucleolin enhanced the translation of mRNAs bearing the G-rich motif, since silencing nucleolin did not change target mRNA stability, but decreased the size of polysomes forming on target transcripts and lowered the abundance of the encoded proteins. In summary, nucleolin binds G-rich sequences in the CR and UTRs of target mRNAs, many of which encode cancer proteins, and enhances their translation.
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影响因子：
5.3
作者：
Galban, Stefanie;Kuwano, Yuki;Gorospe, Myriam
通讯作者：
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影响因子：
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作者：
Al-Souhibani, N.;Al-Ahmadi, W.;Hesketh, J. E.;Blackshear, P. J.;Khabar, K. S. A.
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通讯作者：
Stormo, GD
影响因子：
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作者：
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通讯作者：
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