Anti-invasive effect of gambogic acid in MDA-MB-231 human breast carcinoma cells
Anti-invasive effect of gambogic acid in MDA-MB-231 human breast carcinoma cells
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藤黄酸对MDA-MB-231人乳腺癌细胞的抗侵袭作用
DOI：
10.1139/o08-104
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发表时间：
2008-10-01
影响因子：
2.9
通讯作者：
Guo, Qing-long
中科院分区：
文献类型：
--
作者：
Qi, Qi;Lu, Na;Guo, Qing-long
藤黄酸（GA）在体内外均具有抗肿瘤活性。在本研究中，我们研究了GA在MDA-MB-231人乳腺癌细胞中的抗侵袭作用。结果表明，GA在体外显著抑制细胞的粘附、迁移和侵袭，通过异型粘附实验、创伤迁移实验和小室侵袭实验检测。Western blotting和免疫细胞化学分析结果显示GA可抑制MDA-MB-231细胞基质金属蛋白酶2（MMP-2）和9（MMP-9）的表达。明胶酶谱分析显示GA降低了MMP-2和MMP-9的活性。此外，GA对ERK 1/2和JNK的磷酸化具有抑制作用，而对p38没有影响。综上所述，我们的结果首次证明了GA的抗侵袭特性，并表明它可以作为一种有前途的药物用于治疗癌症转移。
Gambogic acid (GA) has been known to have antitumor activity in vitro and in vivo. In the present study, we investigated the anti-invasive effects of GA in MDA-MB-231 human breast carcinoma cells. The results indicated that GA significantly inhibited the adhesion, migration, and invasion of the cells in vitro tested by the heterotypic adhesion assay, wound migration assay, and chamber invasion assay. Results of Western blotting and immunocytochemistry analysis showed that GA could suppress the expressions of matrix metalloproteinase 2 (MMP-2) and 9 (MMP-9) in MDA-MB-231 cells. Furthermore, gelatin zymography revealed that GA decreased the activities of MMP-2 and MMP-9. Additionally, GA exerted an inhibitory effect on the phosphorylation of ERK1/2 and JNK, while it had no effect on p38. Taken together, our results demonstrated the anti-invasive property of GA for the first time and indicated it could serve as a promising drug for the treatment of cancer metastasis.