PROLACTIN RECEPTORS IN LIVER, KIDNEY, AND GILL OF THE TILAPIA (OREOCHROMIS-MOSSAMBICUS) - CHARACTERIZATION AND EFFECT OF SALINITY ON SPECIFIC BINDING OF IODINATED OVINE PROLACTIN
PROLACTIN RECEPTORS IN LIVER, KIDNEY, AND GILL OF THE TILAPIA (OREOCHROMIS-MOSSAMBICUS) - CHARACTERIZATION AND EFFECT OF SALINITY ON SPECIFIC BINDING OF IODINATED OVINE PROLACTIN
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DOI：
10.1016/0016-6480(90)90226-c
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发表时间：
1990-03-01
影响因子：
2.7
通讯作者：
BERN, HA
中科院分区：
文献类型：
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作者：
DAUDER, S;YOUNG, G;BERN, HA
测定了125I-羊催乳素（oPRL）与成年罗非鱼鳃、肾和肝微粒体组分的特异性结合。组织间特异性结合各不相同，肾膜显示最高值。在肝脏中，oPRL的结合并没有强烈地被罗非鱼催乳素（tPRL 177和tPRL 188）取代，尽管tPRL 177的效力是tPRL 188的6倍。在肾和鳃膜中，两种tPRL的作用是等效的。罗非鱼催乳素表现出较低的效力，在竞争的oPRL结合位点时，利用孕鼠肝膜。罗非鱼生长激素（tGH）和人生长激素（hGH）从肝脏中置换125I-oPRL，以及tPRL 177，但不能很好地被肾或鳃受体识别。两个125I-oPRL结合位点在每个测试的组织中检测到。这些结合位点受到生理调节，因为适应海水导致特异性结合显著减少。
Specific binding of 125I-ovine prolactin (oPRL) to microsomal fractions from gill, kidney, and liver of adult tilapia was determined. Specific binding varied among tissues, the highest values being displayed by kidney membranes. In the liver, the binding of oPRL was not strongly displaced by tilapia prolactins (tPRL177 and tPRL188), although tPRL177 was six times more potent than tPRL188. On the other hand, in kidney and gill membranes, the two tPRLs were equipotent. Tilapia PRLs showed low potency in competing for oPRL-binding sites when pregnant rat liver membranes were utilized. Tilapia growth hormone (tGH) and human growth hormone (hGH) displaced 125I-oPRL from liver as well as did tPRL177 but were not recognized well by renal or branchial receptors. Two 125I-oPRL-binding sites were detected in every tissue tested. These binding sites are subject to physiological regulation since adaptation to seawater resulted in a significant decrease in specific binding.