Inverse method 3-D reconstruction of localized in vivo fluorescence -: Application to Sjogren syndrome
Inverse method 3-D reconstruction of localized in vivo fluorescence -: Application to Sjogren syndrome
复制标题
DOI：
10.1109/2944.796313
复制
发表时间：
1999-07-01
影响因子：
4.9
通讯作者：
Gandjbakhche, AH
中科院分区：
文献类型：
--
作者：
Chernomordik, V;Hattery, D;Gandjbakhche, AH
特异性荧光标记细胞表面标记物的发展为组织变化的特异性和定量无创诊断提供了可能。我们正在开发一种荧光扫描成像系统，可以对干燥综合征（SS）进行“无创光学活检”，这可能取代目前使用的组织学活检。SS的诊断是基于定量的局部预先给药荧光抗体的数量，特异性结合淋巴细胞浸润小唾液腺。我们打算扫描下唇，并且对于扫描的每个位置，使用电荷耦合器件（CCD）相机生成二维（2-D）荧光图像。我们之前已经表明，我们的漫射荧光光子迁移理论可以充分预测嵌入在类组织幽灵中不同深度的一个和两个荧光目标的位置和强度。基于我们的理论发现，用c++编写了一个逆重建算法，并使用二维图像来预测嵌入荧光团的强度和位置。然而，由于存在大量变量，其中包括组织在激发和发射波长处的光学特性，以及未知数量的荧光团目标的位置和强度，因此最终结果的有效性取决于假设（如目标数量）和光学参数的输入值。我们的研究结果表明，每次扫描重建的荧光团目标数量限制在两个，并且至少需要在激发波长处的先验散射系数才能获得良好的结果。后者可以通过测量提供吸收系数和散射系数乘积的激发波长处的空间分辨漫反射来获得。
The development of specific fluorescently labeled cell surface markers have opened the possibility of specific and quantitative noninvasive diagnosis of tissue changes. We are developing a fluorescence scanning imaging system that can perform a "noninvasive optical biopsy" of the Sjogren syndrome (SS) which may replace the currently used histological biopsy. The diagnosis of SS is based on the quantification of the number of topical preadministered fluorescent antibodies which specifically bind to the lymphocytes infiltrating the minor salivary glands. We intend to scan the lower lip, and for each position of the scan, generate a two-dimensional (2-D) image of fluorescence using a charge-coupled del ice (CCD) camera. We have shown previously that our diffuse fluorescent photon migration theory predicts adequately the positions and strengths of one and two fluorescent targets embedded at different depths in tissue-like phantoms, An inverse reconstruction algorithm based on our theoretical findings has been written in C++ and uses 2-D images to predict the strength and location of embedded fluorophores. However, due to large numbers of variables, which include the optical properties of the tissue at the excitation and emission wavelengths, and the positions and strengths of an unknown number of fluorophore targets, the validity of the final result depends on assumptions (such as the number of targets) and the input values for the optical parameters. Our results show that the number of fluorophore targets reconstructed for each scan is limited to two, and at least the scattering coefficient at the excitation wavelength is needed a priori to obtain good results. The latter can be obtained by measurements of spatially resolved diffuse reflectance at the excitation wavelength that provides the product of the absorption and scattering coefficients.