Bioorthogonal Photoreactive Surfaces for Single-Cell Analysis of Intercellular Communications
Bioorthogonal Photoreactive Surfaces for Single-Cell Analysis of Intercellular Communications
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DOI：
10.1021/jacs.2c07321
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发表时间：
2022-09-20
影响因子：
15
通讯作者：
Okamoto，Akimitsu
中科院分区：
文献类型：
--
作者：
Kosaka，Takahiro;Yamaguchi，Satoshi;Okamoto，Akimitsu
构建异质细胞的单细胞对的方法吸引了人们的注意，因为它们在细胞生物学和医学应用中的潜力，用于分析个体细胞间的通信，如免疫和神经突触相互作用。用于细胞锚定的光活化基底表面是实现单细胞配对的有前途的工具。然而，光活化单一类型的细胞锚定部分的常规表面限制了细胞对类型的组合及其应用。我们开发了一种光响应材料，包括生物正交光反应部分和非细胞粘附亲水聚合物。材料涂覆的表面允许在特定时间响应于光与各种细胞锚定分子缀合，并因此在限定区域实现各种细胞的光诱导锚定。使用该平台表面，在平坦基底表面上构建癌细胞和自然杀伤（NK）细胞对的阵列，并且通过在单细胞水平上与NK细胞的细胞毒性相互作用来监测癌细胞的动态形态变化。光反应表面是一种有用的工具，用于基于图像的研究各种细胞类型之间的通信。
Methods to construct single-cell pairs of heterogeneous cells attract attention because of their potential in cell biological and medical applications for analyzing individual intercellular communications such as immune and nerve synaptic interactions. Photoactivatable substrate surfaces for cell anchoring are promising tools to achieve single-cell pairing. However, conventional surfaces that photoactivate a single type of cell anchoring moiety restrict the combination of cell pair types and their applications. We developed a photoresponsive material comprising a bioorthogonal photoreactive moiety and non-cell adhesive hydrophilic polymer. The material-coated surface allows conjugation with various cell anchoring molecules in response to light at specific timings and consequently achieves light-induced anchoring of a variety of cells at defined regions. Using the platform surface, an array of cancer cell and natural-killer (NK) cell pairs was constructed on a flat substrate surface and the dynamic morphological changes of the cancer cells were monitored by cytotoxic interaction with NK cells at a single-cell level. The photoreactive surface is a useful tool for image-based investigation of the communications between a variety of cell types.