Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120.
Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120.
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DOI：
10.1073/pnas.85.9.3198
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发表时间：
1988-05
影响因子：
11.1
通讯作者：
James R. Rusche;K. Javaherian;Charlene MCDANALt;J. Petro;Debra L. Lynn;R. Grimaila;Alphonse J. LANGLOISt;Robert C. Gallo;P. Fischinger;Dani P. BOLOGNESIt;SCOTr D. Putney;Thomas J. MATTHEWSt
中科院分区：
文献类型：
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作者：
James R. Rusche;K. Javaherian;Charlene MCDANALt;J. Petro;Debra L. Lynn;R. Grimaila;Alphonse J. LANGLOISt;Robert C. Gallo;P. Fischinger;Dani P. BOLOGNESIt;SCOTr D. Putney;Thomas J. MATTHEWSt
含有完整包膜gp 160或包膜中心部分PB 1的重组人免疫缺陷病毒（HIV）蛋白的抗血清可抑制培养物中病毒感染细胞的融合。这种融合抑制是HIV变异体特异性的，即抗gp 160-IIIB抑制分离的HTLV-IIIB感染细胞的融合，但不抑制分离的HTLV-IIIRF感染细胞的融合。抗gp 160和抗PB 1两者的融合抑制活性均通过加入PB 1蛋白而被完全阻断。24个氨基酸的肽（RP 135，氨基酸307-330）完全阻断两种抗血清的融合抑制活性，并且还阻断来自感染HTLV-IIIB的黑猩猩的血清的活性。因此，激发融合抑制抗体的主要表位位于gp 120的中心部分。
Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of isolate HTLV-IIIB-infected cells but not of isolate HTLV-IIIRF-infected cells. Both anti-gp160 and anti-PB1 are completely blocked in fusion inhibition activity by the addition of PB1 protein. A 24-amino acid peptide (RP135, amino acids 307-330) completely blocks fusion inhibition activity of both antisera and also blocks the activity of serum from a chimpanzee infected with HTLV-IIIB. Thus, the principal epitope that elicits fusion-inhibiting antibodies is located in the central portion of gp120.