Differences of genetic diversity and antibiotics susceptibility of Pseudomonas aeruginosa isolated from hospital, river and coastal seawater
Differences of genetic diversity and antibiotics susceptibility of Pseudomonas aeruginosa isolated from hospital, river and coastal seawater
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DOI：
10.1111/j.1758-2229.2010.00178.x
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发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Suzuki, Satoru
中科院分区：
文献类型：
--
作者：
Nonaka, Lisa;Inubushi, Ayumi;Suzuki, Satoru
P> 铜绿假单胞菌是一种机会性病原体，在自然环境中普遍存在。然而，关于临床和环境分离株之间差异的细节尚未得到足够的报道。在这项研究中，我们定义了临床、河流和沿海海水分离株中海洋特异性基因组的存在和不同的药物敏感性。采用西曲溴铵卡那霉素萘啶酸琼脂培养基，42℃培养分离铜绿假单胞菌，是从天然水生样品中特异性筛选该菌的方法。脉冲场凝胶电泳分析表明，不同环境地点的铜绿假单胞菌遗传变异水平不同。脉冲场凝胶电泳也显示沿海水域的铜绿假单胞菌多样性较低；不同采样点分离出的沿海菌株紧密分布在同一簇中。与临床分离株相比，大多数水生分离株对大多数测试药物敏感，对帕尼培南“中间”，这表明抗生素的临床使用对耐药铜绿假单胞菌的出现有显着影响。
P>Pseudomonas aeruginosa is an opportunistic pathogen, and ubiquitously found in natural environments. However, details on difference between clinical and environmental isolates have not been reported enough. In this study, we defined existence of marine specific genogroup and different drug susceptibility among isolates from clinical, river and coastal seawaters. Pseudomonas aeruginosa were isolated by using cetrimide kanamycin nalidixic acid agar media and incubation at 42 degrees C, which was specific selection method of this bacterium from the natural aquatic samples. Pulse field gel electrophoresis analysis showed that the levels of genetic variation within P. aeruginosa were different among environmental sites. Pulse field gel electrophoresis also showed a lower diversity within P. aeruginosa in the coastal waters; and coastal strains isolated different sampling points were positioned closely in the same cluster. Most of the aquatic isolates were sensitive to most of the drugs tested and 'intermediate' to panipenem on the contrast to the clinical isolates, suggesting that the clinical use of antibiotics affect significantly to the emergence of the drug-resistant P. aeruginosa.