Electrophysiological properties of mitogen-expanded adult rat spinal cord and subventricular zone neural precursor cells
Electrophysiological properties of mitogen-expanded adult rat spinal cord and subventricular zone neural precursor cells
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DOI：
10.1006/exnr.1999.7078
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发表时间：
1999-07-01
影响因子：
5.3
通讯作者：
Magnuson, DSK
中科院分区：
文献类型：
--
作者：
Liu, RH;Morassutti, DJ;Magnuson, DSK
从哺乳动物中枢神经系统分离的生长因子扩增的神经前体细胞可以分化为神经元和胶质细胞。虽然这些神经前体细胞的形态学和神经化学的发展已被调查，很少有人注意到他们的电生理。本研究探讨了成年大鼠脊髓（SC）和脑室下区（SVZ）神经前体细胞衍生的神经元和胶质细胞的电生理特性。将细胞在含有表皮生长因子和/或成纤维细胞生长因子-2的培养基中培养。在至少两次传代后，将神经前体细胞球接种在包被的盖玻片上，并在培养物中维持长达6周。使用标准电流钳技术进行全细胞膜片记录。在SC和SVZ细胞接种后数小时内观察到不成熟的动作电位。输入电阻和时间常数在电镀后的第一周内下降，并且在以后的时间内没有发现进一步的变化。然而，在类似的时间后，接种，SVZ细胞具有较低的输入电阻和较短的时间常数相比，SC细胞。SVZ细胞也有较高的静息膜电位和较小的超极化后比SC细胞，尽管没有显着差异的动作电位的幅度。无论是SC还是SVZ细胞都不能引起一个以上的动作电位，以响应注入电流。虽然所有测试的SC细胞都被谷氨酸去极化，但SVZ细胞对谷氨酸的反应差异很大。这项研究表明，神经前体细胞从SC和SVZ不同的主动和被动的膜特性。似乎SC和SVZ神经元衍生的神经元的电生理发育在所使用的条件下是不完整的。这些观察结果表明，神经前体细胞从不同的解剖位置可能是生理上的多样性，并可能表现出一些差异的承诺向神经元或神经胶质细胞的表型。(C)北京：科学出版社.
Growth factor-expanded neural precursor cells isolated from the mammalian central nervous system can differentiate into neurons and glia. Although the morphological and neurochemical development of these neural precursor cells has been investigated, little attention has been paid to their electrophysiology. This study examined the electrophysiological properties of neurons and glia derived from neural precursor cells isolated from the adult rat spinal cord (SC) and subventricular zone (SVZ). Cells were cultured in medium containing epidermal growth factor and/or fibroblast growth factor-2. After at least two passages, spheres of neural precursor cells were plated on coated coverslips and maintained in culture for up to 6 weeks. Whole-cell patch recordings were made using standard current clamp techniques. Immature action potentials were observed within hours of plating for both SC and SVZ cells. Input resistance and time constants decreased over the first week after plating and no further changes were found at later times. At similar times following plating, however, SVZ cells had a lower input resistance and shorter time constant compared to SC cells. SVZ cells also had higher resting membrane potentials and smaller after hyperpolarizations than those of SC cells, despite no significant difference in the amplitude of action potentials. Neither the SC nor the SVZ cells were capable of eliciting more than a single action potential in response to injected current. While all SC cells tested were depolarized by glutamate, the response of SVZ cells to glutamate varied considerably. This study revealed that neural precursor cells from SC and SVZ differ in both active and passive membrane properties. It appears also that the electrophysiological development of SC and SVZ precursor-derived neurons is incomplete under the conditions used. These observations suggest that the neural precursor cells from different anatomical locations may be physiologically diverse and may exhibit some differences in commitment toward neuronal or glial phenotypes. (C) 1999 Academic Press.