Endosomal trafficking pathway regulated by ARA6, a RAB5 GTPase unique to plants.
Endosomal trafficking pathway regulated by ARA6, a RAB5 GTPase unique to plants.
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DOI：
10.4161/sgtp.18299
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Ueda, Takashi
中科院分区：
文献类型：
--
作者：
Ebine, Kazuo;Miyakawa, Naoto;Ueda, Takashi
谱系特异性扩张，其次是功能多样化的关键组成部分，在膜运输，被认为有助于细胞器和膜运输途径的谱系特异性多样化。事实上，最近的比较基因组研究表明，RAB和SNARE分子的特异性扩增独立地发生在进化历史上的各种真核谱系中。然而，这一概念的实验验证是困难的，因为详细的功能分析的RAB和SNARE蛋白独特的特定谱系获得了解新的膜运输途径可能已经演变是必不可少的。最近，我们发现植物特异性的RAB GTd 3，ARA 6，和植物特有的R-SNARE，VAMP 727，介导了拟南芥从内体到质膜的运输途径。虽然在动物中也报道了类似的内体运输途径，但在这些运输系统中起作用的分子机制在动物和植物之间不同。因此，从内体到质膜的运输途径似乎已经在动物和植物系统中独立获得。我们进一步证明了ARA 6介导的运输途径是A. thaliana.这些结果表明，收购一个新的膜运输途径可能与最大限度地提高每种生物体的适应性，在一个谱系特异性的方式。
Lineage-specific expansion, followed by functional diversification of key components that act in membrane trafficking, is thought to contribute to lineage-specific diversification of organelles and membrane trafficking pathways. Indeed, recent comparative genomic studies have indicated that specific expansion of RAB and SNARE molecules occurred independently in various eukaryotic lineages over evolutionary history. However, experimental verification of this notion is difficult, because detailed functional analyses of RAB and SNARE proteins uniquely acquired by specific lineages are essential to understanding how new membrane trafficking pathways may have evolved. Recently, we found that a plant-specific RAB GTPase, ARA6, and a plant-unique R-SNARE, VAMP727, mediate a trafficking pathway from endosomes to the plasma membrane in Arabidopsis thaliana. Although a similar endosomal trafficking pathway was also reported in animals, the molecular machineries acting in these trafficking systems differ between animals and plants. Thus, trafficking pathways from endosomes to the plasma membrane appear to have been acquired independently in animal and plant systems. We further demonstrated that the ARA6-mediated trafficking pathway is required for the proper salt-stress response of A. thaliana. These results indicate that acquisition of a new membrane trafficking pathway may be associated with maximization of the fitness of each organism in a lineage-specific manner.