Olfactory bulb neurons express functional, entrainable circadian rhythms
Olfactory bulb neurons express functional, entrainable circadian rhythms
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DOI：
10.1111/j.0953-816x.2004.03117.x
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发表时间：
2004-02-01
影响因子：
3.4
通讯作者：
Herzog, ED
中科院分区：
文献类型：
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作者：
Granados-Fuentes, D;Saxena, MT;Herzog, ED
昼夜节律起搏器驱动许多日常分子、生理和行为节律。我们研究了大鼠的主嗅球是否是一个功能性的昼夜节律起搏器。长期，多电极记录显示，个人，培养的灯泡神经元表达近24小时振荡的放电率。实时记录的第1阶段的基因活性表明，在灯泡内的细胞群表达同步的节奏开始于胚胎第19天。这种节律性是固有的二尖瓣，而不是颗粒，细胞层，不符合生理温度周期和温度补偿在其期间。然而，去除嗅球对转轮行为没有影响。这些结果表明，单个二尖瓣/簇状细胞是正常情况下相互同步的有能力的昼夜节律起搏器。嗅球内在的基因表达和发射率的每日节律对于昼夜节律运动模式是不需要的，这表明它们参与了典型昼夜节律系统之外的节律。
Circadian pacemakers drive many daily molecular, physiological and behavioural rhythms. We investigated whether the main olfactory bulb is a functional circadian pacemaker in rats. Long-term, multielectrode recordings revealed that individual, cultured bulb neurons expressed near 24-h oscillations in firing rate. Real-time recordings of Period 1 gene activity showed that a population of cells within the bulb expressed synchronized rhythmicity starting on embryonic day 19. This rhythmicity was intrinsic to the mitral, and not the granule, cell layer, entrainable to physiological temperature cycles and temperature compensated in its period. However, removal of the olfactory bulbs had no effect on running wheel behaviour These results indicate that individual mitral/tufted cells are competent circadian pacemakers which normally synchronize to each other The daily rhythms in gene expression and firing rate intrinsic to the olfactory bulb are not required for circadian patterns of locomotion, indicating that they are involved in rhythms outside the canonical circadian system.