INSULIN INHIBITS APOLIPOPROTEIN-B SECRETION IN ISOLATED HUMAN HEPATOCYTES
INSULIN INHIBITS APOLIPOPROTEIN-B SECRETION IN ISOLATED HUMAN HEPATOCYTES
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DOI：
10.1016/0026-0495(91)90109-a
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发表时间：
1991-03-01
影响因子：
9.8
通讯作者：
AMATRUDA, JM
中科院分区：
文献类型：
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作者：
SALHANICK, AI;SCHWARTZ, SI;AMATRUDA, JM
在人肝细胞中研究了胰岛素对载脂蛋白（apo）B分泌的影响。通过胶原酶分散肝脏标本制备新鲜分离的肝细胞，在不存在和存在100 nmol/L胰岛素的情况下，在无血清培养基中孵育2小时。然后通过放射免疫测定法测定培养基中的载脂蛋白B含量。在无胰岛素孵育的肝细胞中，apo B（相对于人低密度脂蛋白[LDL]）的分泌量为125 ± 37 ng/106个细胞/2小时。在胰岛素存在下，apo B分泌减少至83 ± 29 ng/10 6个细胞/2小时（34%抑制，P<0.05）。使用人肝细胞的这些结果与我们实验室先前描述大鼠肝细胞原代培养物中apo B分泌的胰岛素依赖性抑制的数据以及其他人使用人源性肝癌细胞系Hep G2进行的研究一致。我们的结论是，人肝载脂蛋白B的分泌受胰岛素控制。更多的慢性胰岛素暴露的作用需要进一步研究。
The effect of insulin on apolipoprotein (apo) B secretion was investigated in human hepatocytes. Freshly isolated hepatocytes, prepared by collagenase dispersion of liver specimens, were incubated in serum-free media in the absence and presence of 100 nmol/L insulin for 2 hours. The media was then assayed for apo B content by radioimmunoassay. In hepatocytes incubated without insulin, the secretion of apo B (relative to human low-density lipoprotein [LDL]) was 125 ± 37 ng/106cells/2 hours. In the presence of insulin, apo B secretion was reduced to 83 ± 29 ng/106cells/2 hours (34% inhibition,P< .05). These results using human hepatocytes are consistent with previous data from our laboratory describing insulin-dependent inhibition of apo B secretion in primary cultures of rat hepatocytes and studies by others employing the human-derived hepatoma cell line, Hep G2. We conclude that human hepatic apo B secretion is under insulin control. The role of more chronic insulin exposure requires further investigation.