Proximal-distal patterns of transcription factor gene expression during Arabidopsis root development
Proximal-distal patterns of transcription factor gene expression during Arabidopsis root development
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DOI：
10.1093/jxb/erm301
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发表时间：
2008-02-01
影响因子：
6.9
通讯作者：
Dolan, Liam
中科院分区：
文献类型：
--
作者：
Derbyshire, Paul;Drea, Sinead;Dolan, Liam
基因的表达模式可以识别细胞中各自的蛋白质在发育过程中是活跃的。作为确定控制根系发育的遗传网络的一步，已经开发了一种高通量的全安装原位杂交方法，该方法不需要昂贵的设备，并且允许定义137个转录因子基因在年轻发育的根系中的表达模式。137个转录因子中，81.8%在根中表达，18.2%未表达。在根的近端和远端三个区（分生组织、伸长和分化）中，52.6%的基因表达，而只有两个区表达21.2%。8%的基因在单个近端和远端区域表达。细胞特异性基因表达模式也被检测到。这种快速的方法确定了细胞分化的潜在关键调控因子，并为在根发育过程中起作用的大量转录调控因子的表达模式提供了重要的空间信息。
The expression pattern of genes can identify the cells in which the respective proteins are active during development. As a step towards defining the genetic network that controls the development of roots, a high-throughput method of whole-mount in situ hybridization has been developed that does not require expensive equipment and allows the definition of the expression patterns of 137 transcription factor genes in young developing roots. Of the 137 transcription factors, 81.8% were expressed in the root while 18.2% showed no detectable expression. In all three proximal distal zones (meristem, elongation, and differentiation) of the root, 52.6% were expressed whereas 21.2% were expressed in only two zones. Eight percent of the genes were expressed in a single proximal distal zone. Cell-specific gene expression patterns were also detected. This rapid approach identified potential key regulators of cell differentiation and provides important spatial information for the expression patterns of a large number of transcriptional regulators that function during root development.