Detection of hantavirus in bats from remaining rain forest in São Paulo, Brazil.
Detection of hantavirus in bats from remaining rain forest in São Paulo, Brazil.
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DOI：
10.1186/1756-0500-5-690
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发表时间：
2012-12-21
影响因子：
1.8
通讯作者：
Durigon EL
中科院分区：
文献类型：
--
作者：
de Araujo J;Thomazelli LM;Henriques DA;Lautenschalager D;Ometto T;Dutra LM;Aires CC;Favorito S;Durigon EL
巴西发现的重要生物多样性有可能出现新的人畜共患病。世界一些地方的研究表明蝙蝠组织中存在汉坦病毒。在巴西，针对野生动物（啮齿动物）的汉坦病毒研究非常少。因此，我们决定对在啮齿类动物检测出该病毒呈阳性的同一地区捕获的不同种类野生动物的组织进行研究。目前的工作分析了来自巴西圣保罗州比里蒂巴米林市大西洋森林地区的 91 只动物（64 只啮齿动物、19 只负鼠和 8 只蝙蝠）。肺和肾用于RNA提取。通过基于 SYBR-Green 的实时 RT-PCR 筛选样本中汉坦病毒感染的证据。在野生啮齿动物、蝙蝠和负鼠中发现了 16 个阳性样本。在三种有袋动物（Micoureus paraguayanus、Monodelphis ihering 和 Didelphis aurita）以及两种蝙蝠（Diphylla ecaudata 和 Anoura caudifer）的肺和肾中检测到汉坦病毒具有重要意义，因为这些新宿主可能代表重要的病毒储存库。对部分S片段的核苷酸序列分析表明，这些基因与阿拉拉夸拉病毒株更相关。这项工作强调了研究不同动物物种中的汉坦病毒并在该病毒在新宿主中传播并造成严重公共卫生问题之前进行持续监测的重要性。
The significant biodiversity found in Brazil is a potential for the emergence of new zoonoses. Study in some places of the world suggest of the presence to hantavirus in tissues of bats. Researches of hantavirus in wildlife, out rodents, are very scarce in Brazil. Therefore we decided to investigate in tissues of different species of wild animals captured in the same region where rodents were detected positive for this virus. The present work analyzed ninety-one animals (64 rodents, 19 opossums, and 8 bats) from a region of the Atlantic forest in Biritiba Mirin City, São Paulo State, Brazil. Lungs and kidneys were used for RNA extraction. The samples were screened for evidence of hantavirus infection by SYBR-Green-based real-time RT-PCR. Sixteen samples positive were encountered among the wild rodents, bats, and opossums. The detection of hantavirus in the lungs and kidneys of three marsupial species (Micoureus paraguayanus, Monodelphis ihering, and Didelphis aurita) as well in two species of bats (Diphylla ecaudata and Anoura caudifer) is of significance because these new hosts could represent an important virus reservoirs. The analysis of nucleotide sequences of the partial S segment revealed that these genes were more related to the Araraquara virus strains. This work reinforces the importance of studying hantavirus in different animal species and performing a continued surveillance before this virus spreads in new hosts and generated serious problems in public health.