Ultrastructural characterization of interstitial cells of Cajal associated with the submucosal plexus in the proximal colon of the guinea pig
Ultrastructural characterization of interstitial cells of Cajal associated with the submucosal plexus in the proximal colon of the guinea pig
复制标题
豚鼠近端结肠粘膜下神经丛相关 Cajal 间质细胞的超微结构特征
DOI：
10.1007/s00441-011-1312-5
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发表时间：
2012
影响因子：
3.6
通讯作者：
Komuro T
中科院分区：
文献类型：
--
作者：
Tamada H;Komuro T
应用免疫组织化学和电镜技术对豚鼠近端结肠粘膜下神经丛（ICC-SP）相关的Cajal间质细胞（ICC）进行了研究。整体安装拉伸准备与c-Kit免疫组化显示，一些ICC-SP构成了一个密集的细胞网络周围的粘膜下神经丛。在c-Kit和α-平滑肌肌动蛋白免疫染色切片中，在粘膜肌层附近观察到其中一些ICC-SP。超微结构观察首次证实，豚鼠近端结肠ICC-SP保留了多次报道的与胃肠道图尼卡肌层相关的ICC的典型超微结构特征：基膜、小窝、大量线粒体、丰富的中间丝和与同种细胞形成的缝隙连接。最显着的超微结构发现是存在的ICC-SP通过大的间隙连接彼此连接的过程组成的厚束。这些ICC-SP可能参与豚鼠近端结肠的主要粘膜功能，即水和电解质的运输，可能通过它们参与粘膜肌层的自发收缩。
Interstitial cells of Cajal (ICC) associated with the submucosal (submucous) plexus (ICC-SP) in the proximal colon of the guinea pig were studied by immunohistochemistry and electron microscopy. Whole-mount stretch preparations with c-Kit immunohistochemistry revealed that a number of ICC-SP constituted a dense cellular network around the submucosal plexus. Some of these ICC-SP were observed in the vicinity of the muscularis mucosae in sections immunostained for c-Kit and α-smooth muscle actin. Ultrastructural observation demonstrated, for the first time, that ICC-SP of the proximal colon of the guinea pig retained typical ultrastructural characteristics of ICC repeatedly reported in association with the tunica muscularis of the gastrointestinal tract: a basal lamina, caveolae, many mitochondria, abundant intermediate filaments and the formation of gap junctions with the same type of cells. The most remarkable ultrastructural finding was the presence of thick bundles composed of the processes of ICC-SP connected to each other via large gap junctions. These ICC-SP might be involved in the main mucosal functions of the proximal colon of the guinea pig, namely the transportation of water and electrolytes, possibly via their involvement in the spontaneous contractions of the muscularis mucosae.