Prostate-specific membrane antigen-targeted photodynamic therapy induces rapid cytoskeletal disruption
Prostate-specific membrane antigen-targeted photodynamic therapy induces rapid cytoskeletal disruption
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DOI：
10.1016/j.canlet.2010.04.003
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发表时间：
2010-10-01
期刊：
影响因子：
9.7
通讯作者：
Berkman, Clifford E.
中科院分区：
文献类型：
--
作者：
Liu, Tiancheng;Wu, Lisa Y.;Berkman, Clifford E.
前列腺特异性膜抗原（PSMA），一种已建立的前列腺癌的酶生物标志物，作为成像和治疗应用的靶点引起了相当大的关注。我们的目的是确定PSMA靶向光动力疗法（PDT）对前列腺癌细胞骨架网络的影响。靶向PSMA的PDT导致LNCaP细胞的细胞质中的微管（α-/β-微管蛋白）、微丝（肌动蛋白）和中间丝（细胞角蛋白8/18）的快速破坏。细胞质微管的崩溃和后来的α-/β-微管蛋白的核转位是最引人注目的交替。这些细胞骨架网络的早期变化可能部分参与了细胞死亡的启动。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Prostate-specific membrane antigen (PSMA), an established enzyme-biomarker for prostate cancer, has attracted considerable attention as a target for imaging and therapeutic applications. We aimed to determine the effects of PSMA-targeted photodynamic therapy (PDT) on cytoskeletal networks in prostate cancer cells. PSMA-targeted PDT resulted in rapid disruption of microtubules (alpha-/beta-tubulin), microfilaments (actin), and intermediate filaments (cytokeratin 8/18) in the cytoplasm of LNCaP cells. The collapse of cytoplasmic microtubules and the later nuclear translocation of alpha-/beta-tubulin were the most dramatic alternation. It is likely that these early changes of cytoskeletal networks are partly involved in the initiation of cell death. (C) 2010 Elsevier Ireland Ltd. All rights reserved.