ACTIVATION OF THE PP60C-SRC KINASE DURING DIFFERENTIATION OF MONOMYELOCYTIC CELLS-INVITRO
ACTIVATION OF THE PP60C-SRC KINASE DURING DIFFERENTIATION OF MONOMYELOCYTIC CELLS-INVITRO
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DOI：
10.1002/j.1460-2075.1986.tb04270.x
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发表时间：
1986-04-01
期刊：
影响因子：
11.4
通讯作者：
GESSLER, M
中科院分区：
文献类型：
--
作者：
BARNEKOW, A;GESSLER, M
原癌基因c-src是劳斯肉瘤病毒（RSV）转化基因v-src的细胞同源物，以组织特异性和年龄依赖性方式表达。其生理功能，虽然仍然未知，似乎是更密切相关的分化过程比增殖过程。为了获得更多的信息，c-src基因在细胞中的生理作用，我们已经研究了分化依赖性的改变使用人HL-60白血病细胞系作为模型系统。12-O-十四烷酰佛波醇-13-乙酸酯（TPA）和二甲基亚砜（DMSO）诱导HL-60细胞向单核细胞和粒细胞分化与pp 60 c-src酪氨酸（DMSO）的激活有关，与pp 60 c-src酪氨酸激酶的激活有关，但与c-src基因表达的增加无关。对照实验排除TPA和DMSO本身与pp 60 c-src激酶的相互作用。
The proto-oncogene c-src, the cellular homolog of the Rous sarcoma virus (RSV) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. Its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. To obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human HL-60 leukaemia cell line as a model system. Induction of monocytic and granulocytic differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) and dimethylsulfoxide (DMSO) is associated with an activation of the pp60c-src tyrosine (DMSO) is associated with an activation of the pp60c-src tyrosine kinase, but not with increased c-src gene expression. Control experiments exclude an interaction of TPA and DMSO themselves with the pp60c-src kinase.