The signal from the initiation of meiotic recombination to the first division of meiosis
The signal from the initiation of meiotic recombination to the first division of meiosis
复制标题
DOI：
10.1128/ec.3.3.598-609.2004
复制
发表时间：
2004-06-01
期刊：
影响因子：
--
通讯作者：
Lee, KE
中科院分区：
文献类型：
--
作者：
Malone, RE;Haring, SJ;Lee, KE
减数分裂中发生的两个独特事件是高水平的遗传重组和减数分裂。我们以前的工作表明，REC 102，REC 104，REC 114，和RAD 50基因，启动减数分裂重组所需的酿酒酵母，需要适当的时间的第一次减数分裂（MI）分裂。如果这些基因不存在，MI分裂实际上在更早的时间开始。本文证明减数分裂重组基因MER 2/REC 107、SPO 11和MRE 2以及联会复合体基因HOP 1和RED 1也是MI分裂正常延迟所必需的。rec 103/ski 8突变体与野生型细胞同时开始MI分裂。我们的数据表明，在Rec（-）突变体中，减数分裂前S期的时间和第一次分裂的时间之间没有明显的相关性。rec 102或rec 104突变的细胞在野生型细胞之前形成MI纺锤体，这表明起始信号在纺锤体形成之前起作用。不需要RAD 9或RAD 24对信号进行分频，这会延迟第一分频。RAD 24突变体中MI分裂的时间表明，粗线期检查点在Rec（+）细胞中不活跃，并表明重组和野生型细胞中MI分裂之间的协调可能主要由于起始信号而发生。最后，重组起始信号的至少一个靶是NDT 80基因，其是第一次分裂所需的中期减数分裂基因表达的转录调节因子。
Two of the unique events that occur in meiosis are high levels of genetic recombination and the reductional division. Our previous work demonstrated that the REC102, REC104, REC114, and RAD50 genes, required to initiate meiotic recombination in Saccharomyces cerevisiae, are needed for the proper timing of the first meiotic (MI) division. If these genes are absent, the MI division actually begins at an earlier time. This paper demonstrates that the meiotic recombination genes MER2/REC107, SPO11, and MRE2 and the synaptonemal complex genes HOP1 and RED1 are also required for the normal delay of the MI division. A rec103/ski8 mutant starts the MI division at the same time as in wild-type cells. Our data indicate no obvious correlation between the timing of premeiotic S phase and the timing of the first division in Rec(-) mutants. Cells with rec102 or rec104 mutations form MI spindles before wild-type cells, suggesting that the initiation signal acts prior to spindle formation. Neither RAD9 nor RAD24 is needed to transduce the signal, which delays the first division. The timing of the MI division in RAD24 mutants indicates that the pachytene checkpoint is not active in Rec(+) cells and suggests that the coordination between recombination and the MI division in wild-type cells may occur primarily due to the initiation signal. Finally, at least one of the targets of the recombination initiation signal is the NDT80 gene, a transcriptional regulator of middle meiotic gene expression required for the first division.