Differential effects of sodium salicylate on current-evoked firing of pyramidal neurons and fast-spiking interneurons in slices of rat auditory cortex
Differential effects of sodium salicylate on current-evoked firing of pyramidal neurons and fast-spiking interneurons in slices of rat auditory cortex
复制标题
水杨酸钠对大鼠听觉皮层切片中锥体神经元和快速尖峰中间神经元电流诱发放电的不同影响
DOI：
10.1016/j.heares.2009.03.007
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发表时间：
2009-07-09
期刊：
影响因子：
2.8
通讯作者：
Chen, Lin
中科院分区：
文献类型：
--
作者：
Su, Yan-Yan;Luo, Bin;Chen, Lin
水杨酸钠(SS)可穿透血脑屏障，靶向中枢听觉系统的神经元。了解SS如何改变不同类型中枢听神经元的功能行为，将有助于深入了解SS诱导耳鸣的神经机制。在这里，我们报道了SS对幼年大鼠听皮层脑片II/III层锥体神经元和快峰中间神经元电流诱发放电的不同影响(P12-P19)。根据全细胞膜片钳技术记录到的电流诱发放电的特征模式和形态特征来识别这两种神经元类型。用1.4 mM SS灌流脑片后，锥体神经元诱发动作电位所需的阈值电流无明显变化(68.96+/-10.68pA比70.39+/-12.14pA，n=7，P>；0.05)，而快速放电中间神经元的阈值电流显著增加(56.9+/-13.69pA比74.04+/-15.73pA，n=7，P&t；0.05)。药物灌流对锥体神经元电流诱发放电频率无明显影响(-2.43+/-7.07%，n=14，P>；0.05)，但对快速放电中间神经元电流诱发放电频率有明显抑制作用(-49.88±-10.39%，n=14，P>；0.05)。我们的结果提示，GABA能和抑制性快速放电中间神经元的功能受损可能是SS提高中枢听觉系统兴奋性从而产生耳鸣的途径之一。(C)2009爱思唯尔B.V.保留所有权利。
Sodium salicylate (SS) can penetrate the blood-brain barrier to target neurons in the central auditory system. Understanding how SS alters functional behaviors of different types of central auditory neurons will provide insights into the neural mechanisms of SS-induced tinnitus. Here, we report the differential effects of SS on current-evoked firing of pyramidal neurons and fast-spiking interneurons in layer II/III of auditory cortex slices in young rats (P12-P19). The two neuronal types were identified according to their characteristic patterns of current-evoked firing as recorded with whole-cell patch-clamp techniques and by their morphological features. Following perfusion of the brain slice with 1.4 mM SS, the threshold current needed to evoke an action potential remained unchanged for pyramidal neurons (68.96 +/- 10.68 pA vs 70.39 +/- 12.14 pA, n = 7, P > 0.05), but significantly increased for fast-spiking interneurons (56.9 +/- 13.69 pA vs 74.04 +/- 15.73 pA, n = 7, P < 0.05). The drug perfusion caused no significant change in current-evoked firing rates in pyramidal neurons (-2.43 +/- 7.07%, n = 14, P > 0.05); however, it drastically and reversibly depressed those in fast-spiking interneurons by up to -49.88 +/- 10.39% (n = 14, P < 0.05). Our results suggest that functionally impairing fast-spiking interneurons, which are GABAergic and inhibitory, is probably one of the pathways through which SS raises excitability in the central auditory system and consequently produces tinnitus. (C) 2009 Elsevier B.V. All rights reserved.