Cloning, expression, and purification of a catalytic fragment of Moloney murine leukemia virus reverse transcriptase: crystallization of nucleic acid complexes.
Cloning, expression, and purification of a catalytic fragment of Moloney murine leukemia virus reverse transcriptase: crystallization of nucleic acid complexes.
复制标题
莫洛尼鼠白血病病毒逆转录酶催化片段的克隆、表达和纯化：核酸复合物的结晶。
DOI：
10.1002/pro.5560070711
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Georgiadis,MM
中科院分区：
文献类型：
--
作者：
Sun,D;Jessen,S;Liu,C;Liu,X;Najmudin,S;Georgiadis,MM
Reverse transcriptase is an essential retroviral enzyme that uses RNA‐ and DNA‐directed DNA polymerase activities as well as an RNaseH activity to synthesize a double‐stranded DNA copy of the single‐stranded RNA genome. In an effort to obtain high‐resolution structural information regarding the polymerase active site of reverse transcriptase, we have pursued studies on a catalytic fragment from Moloney murine leukemia virus reverse transcriptase. DNA encoding the catalytic fragment, defined originally by limited proteolytic digestion, has been cloned, and the protein has been expressed and purified fromEscherichia coli. The fragment obtained by limited proteolytic digestion and the bacterially expressed fragment retain polymerase activity. Crystallization studies involving nucleic acid complexes with a catalytic fragment from both sources are reported, including variables screened to improve crystals and cryocooling. Three crystal forms of catalytic fragment‐nucleic acid complexes have been characterized, which all contain at least two protein molecules in the asymmetric unit. As isolated, the catalytic fragment is monomeric. This analysis indicates that the enzyme dimerizes in the presence of nucleic acid.
