Donor-host mitochondrial compatibility improves efficiency of bovine somatic cell nuclear transfer.
Donor-host mitochondrial compatibility improves efficiency of bovine somatic cell nuclear transfer.
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供体-宿主线粒体相容性提高牛体细胞核移植效率
DOI：
10.1186/1471-213x-10-31
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发表时间：
2010-03-19
影响因子：
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通讯作者：
Zeng, Fanyi
中科院分区：
文献类型：
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作者：
Yan, Zhong-hai;Zhou, Yi-ye;Fu, Jing;Jiao, Fei;Zhao, Lei-wen;Guan, Peng-fei;Huang, Shu-zhen;Zeng, Yi-tao;Zeng, Fanyi
核质体和细胞质之间的相互作用对体细胞核移植（SCNT）的效率起着重要作用，但其潜在机制仍不清楚。人们普遍认为，在核移植胚胎中，基因表达的重编程是由表观遗传机制诱导的，不涉及DNA序列的修饰。在牛中，具有各种线粒体 DNA (mtDNA) 单倍型的卵母细胞通常具有不同的 ATP 含量，可以进一步影响胚胎体外生产的效率。由于线粒体DNA来自SCNT过程中的受体卵母细胞，并受到供体核中基因的调控，因此它是研究SCNT中供体核与宿主卵母细胞之间相互作用的完美模型。我们研究了 SCNT 产生的重建牛胚胎的体外发育是否会受到卵母细胞和供体细胞之间 mtDNA 单倍型兼容性的影响。来自同型 A-A 或 B-B 的胚胎在囊胚阶段表现出比异型 A-B 或 B-A 组合显着更高的发育能力。同型 SCNT 组的着床后发育能力、妊娠第 90 天的妊娠率以及足月产率均高于异型组。此外，同型和异型 SCNT 胚胎在全基因组和多能性相关基因（Oct-4、Sox-2、Nanog）上显示出不同的组蛋白 3-赖氨酸 9 (H3K9) 甲基化模式。组蛋白和 DNA 甲基化均表明同型 SCNT 囊胚比异型 SCNT 囊胚具有更成功的表观遗传不对称模式，这表明更完整的核重编程。这可能是由于它们的表观遗传模式的变异性和对核重编程的反应造成的。这表明供体细胞和宿主卵母细胞之间mtDNA单倍型的相容性可以显着影响SCNT中重建胚胎的发育能力，并且可能包括表观遗传机制。
The interaction between the karyoplast and cytoplast plays an important role in the efficiency of somatic cell nuclear transfer (SCNT), but the underlying mechanism remains unclear. It is generally accepted that in nuclear transfer embryos, the reprogramming of gene expression is induced by epigenetic mechanisms and does not involve modifications of DNA sequences. In cattle, oocytes with various mitochondrial DNA (mtDNA) haplotypes usually have different ATP content and can further affect the efficiency of in vitro production of embryos. As mtDNA comes from the recipient oocyte during SCNT and is regulated by genes in the donor nucleus, it is a perfect model to investigate the interaction between donor nuclei and host oocytes in SCNT. We investigated whether the in vitro development of reconstructed bovine embryos produced by SCNT would be influenced by mtDNA haplotype compatibility between the oocytes and donor cells. Embryos from homotype A-A or B-B showed significantly higher developmental ability at blastocyst stages than the heterotype A-B or B-A combinations. Post-implantation development ability, pregnancy rate up to day 90 of gestation, as well as percent of term births were higher in the homotype SCNT groups than in the heterotype groups. In addition, homotype and heterotype SCNT embryos showed different methylation patterns of histone 3-lysine 9 (H3K9) genome-wide and at pluripotency-related genes (Oct-4, Sox-2, Nanog). Both histone and DNA methylation show that homotype SCNT blastocysts have a more successful epigenetic asymmetry pattern than heterotype SCNT blastocysts, which indicates more complete nuclear reprogramming. This may result from variability in their epigenetic patterns and responses to nuclear reprogramming. This suggests that the compatibility of mtDNA haplotypes between donor cells and host oocytes can significantly affect the developmental competence of reconstructed embryos in SCNT, and may include an epigenetic mechanism.