Development of collagen/polydopamine complexed matrix as mechanically enhanced and highly biocompatible semi-natural tissue engineering scaffold
Development of collagen/polydopamine complexed matrix as mechanically enhanced and highly biocompatible semi-natural tissue engineering scaffold
复制标题
开发胶原/聚多巴胺复合基质作为机械增强且高度生物相容的半天然组织工程支架
DOI：
10.1016/j.actbio.2016.10.017
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发表时间：
2017-01-01
影响因子：
9.7
通讯作者：
Gu, Zhipeng
中科院分区：
文献类型：
--
作者：
Hu, Yang;Dan, Weihua;Gu, Zhipeng
为了改善胶原蛋白基质的力学性能和生物相容性，采用多巴胺自聚合法对猪脱细胞真皮基质（PADM）进行改性，并结合胶原蛋白固定化，以提高PADM的生物学、力学和物理化学性能。机理研究表明，多巴胺在PADM表面的聚合可以通过控制PADM胶原纤维中酚羟基（C-OH）与氮原子（N-C=O）之间形成氢键的数量来调控。PDA与PADM之间的表面相互作用研究表明，PDA-PADM体系具有更好的力学性能、热稳定性、表面亲水性，且多巴胺包覆后的PADM保持了结构完整性。将胶原（COL）固定于新鲜PDA-PADM上，制备胶原-PDA-PADM（COL-PDA-PADM）复合支架。MTT法和CLSM观察表明，COL-PDA-PADM的生物相容性和细胞粘附性均优于纯PADM和未包被多巴胺的COL-PADM，从而证明了PDA作为中间层的有效性。同时，通过体内实验研究COL-PDA-PADM对碱性成纤维细胞生长因子（bFGF）和血管内皮生长因子（VEGF）的表达。结果表明COL-PDA-PADM能有效促进bFGF和VEGF的表达，从而促进硬脑膜的修复。总之，本工作为开发具有高生物相容性和良好力学性能的半天然组织工程支架提供了新的见解。重要性声明获得具有高生物相容性和良好力学性能的支架仍然是组织工程中最具挑战性的问题之一。为了具有优异的体外和体内性能，支架需要具有与天然细胞外基质（例如基于胶原的基质）相似的机械和生物学性质。利用表面自交联和涂层策略，我们成功地从猪脱细胞真皮基质（PADM）、聚多巴胺和胶原蛋白中获得了一种具有优良生物学和机械性能的新型半天然平台。结果证实，该支架平台具有非常优异的细胞性能和非常小的体内毒性/副作用。因此，这种半天然支架可能是一个合适的组织工程平台，这种策略将进一步有助于开发更强大的支架。(C)2016 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
To improve the mechanical properties and biocompatibility of collagen I matrix, a novel and facile strategy was developed to modify porcine acellular dermal matrix (PADM) via dopamine self-polymerization followed by collagen immobilization to enhance the biological, mechanical and physicochemical properties of PADM. Mechanism study indicated that the polymerization of dopamine onto PADM surface could be regulated by controlling the amount of hydrogen bonds forming between phenol hydroxyl (C-OH) and nitrogen atom (N-C=O) within collagen fibers of PADM. The investigations of surface interactions between PDA and PADM illustrated that PDA-PADM system yielded better mechanical properties, thermal stability, surface hydrophilicity and the structural integrity of PADM was maintained after dopamine coating. Furthermore, collagen (COL) was immobilized onto the fresh PDA-PADM to fabricate the collagen-PDA-PADM (COL-PDA-PADM) complexed scaffold. The MTT assay and CLSM observation showed that COL-PDA-PADM had better biocompatibility and higher cellular attachment than pure PADM and COL-PADM without dopamine coating, thus demonstrating the efficacy of PDA as the intermediate layer. Meanwhile, the expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) of COL-PDA-PADM were investigated by an in vivo study. The results revealed that COL-PDA-PADM could effectively promote bFGF and VEGF expression, possibly leading to enhancing the dura repairing process. Overall, this work contributed a new insight into the development of a semi natural tissue engineering scaffold with high biocompatibility and good mechanical properties.Statement of SignificanceObtaining scaffolds with high biocompatibility and good mechanical properties is still one of the most challenging issues in tissue engineering. To have excellent in vitro and in vivo performance, scaffolds are desired to have similar mechanical and biological properties as the natural extracellular matrix, such as collagen based matrix. Utilizing the surface self-crosslinking and coating strategy, we successfully obtained a novel semi-natural platform with excellent biological and mechanical properties from porcine acellular dermal matrix (PADM), polydopamine and collagen. The results confirmed that this scaffold platform has very excellent cellular performance and very little toxicity/side effects in vivo. Therefore, this semi-natural scaffold may be an appropriate platform for tissue engineering and this strategy would further help to develop more robust scaffolds. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.