Archaeal community dynamics and detection of ammonia-oxidizing archaea during composting of cattle manure using culture-independent DNA analysis
Archaeal community dynamics and detection of ammonia-oxidizing archaea during composting of cattle manure using culture-independent DNA analysis
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DOI：
10.1007/s00253-011-3153-2
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发表时间：
2011-02
影响因子：
5
通讯作者：
Nozomi Yamamoto;Ryoki Asano;Hiroki Yoshii;K. Otawa;Y. Nakai
中科院分区：
文献类型：
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作者：
Nozomi Yamamoto;Ryoki Asano;Hiroki Yoshii;K. Otawa;Y. Nakai
堆肥过程是在有氧条件下进行的，涉及细菌，古细菌和真菌。堆肥中古菌群落的多样性知之甚少，尽管它们可能在甲烷产生和氨氧化中发挥重要作用。本研究利用古细菌16S rRNA基因克隆文库对牛粪堆肥过程中古细菌群落动态进行了分析。结果表明，产甲烷古菌（产甲烷菌）和氨氧化古菌（AOA）可能是整个堆肥过程中的优势微生物。从第2天开始，群落主要由甲烷小体样序列和甲烷八叠球菌样序列组成，从第6天到第30天，亚硝化单胞菌样序列逐渐增多。嗜热菌类物种可以适应温度升高或营养流失。变性梯度凝胶电泳分析表明，优势amoA基因序列的同源性为99%，从不同的堆肥设施获得的相同，与nitrososphaera gargensis。这些数据表明，AOA可能在几种堆肥实践中的氨氧化中发挥作用。我们的研究结果提供了有关古菌群落动态的基本信息，这将有助于了解堆肥中的集体微生物群落。
The composting process is carried out under aerobic conditions involving bacteria, archaea, and fungi. Little is known about the diversity of archaeal community in compost, although they may play an important role in methane production and ammonia oxidation. In the present study, archaeal community dynamics during cattle manure composting were analyzed using a clone library of the archaeal 16S rRNA gene. The results indicated that methane-producing archaea (methanogen) and ammonia-oxidizing archaea (AOA) may be the dominant microbes throughout the composting. The community consisted primarily ofMethanocorpusculum-like andMethanosarcina-like sequences until day 2, while the number ofCandidatusNitrososphaera-like sequences increased from day 6 to day 30.Methanosarcina thermophila-like sequences were dominant from day 2, suggesting thatM. thermophila-like species can adapt to increasing temperature or nutrient loss. A denaturant gradient gel electrophoresis analysis of the archaealamoAgenes revealed that the dominantamoAgene sequence with 99% homology to that ofCandidatusNitrososphaera gargensis was identical to those obtained from a different composting facility. These data suggested that AOA may play a role in ammonia oxidation in several composting practices. Our results provide fundamental information regarding archaeal community dynamics that will help in understanding the collective microbial community in compost.