SYNERGY AMONG RESPONDING LYMPHOID CELLS IN THE ONE-WAY MIXED LYMPHOCYTE REACTION
SYNERGY AMONG RESPONDING LYMPHOID CELLS IN THE ONE-WAY MIXED LYMPHOCYTE REACTION
复制标题
单向混合淋巴细胞反应中反应淋巴细胞之间的协同作用
DOI：
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发表时间：
1974
影响因子：
15.3
通讯作者：
R. Walford
中科院分区：
文献类型：
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作者：
W. Tittor;M. Gerbase‐DeLima;R. Walford
目前的研究表明，胸腺依赖性淋巴细胞的两个亚群可能在小鼠混合淋巴细胞反应（MLR）中协同作用。一个亚群在年轻成人胸腺中得到很好的体现，而另一种亚群则在淋巴结中得到很好的体现。为了获得最佳协同作用，两个细胞群必须与刺激细胞同种异体。用丝裂霉素-C 预处理任一群体都消除了协同作用。抗θ血清消除了淋巴细胞的MLR反应和协同活性。这两个胸腺依赖性亚群都存在于脾脏中，并且在注射到受辐射小鼠体内时表现出不同的迁移模式：一个群体进入受辐射小鼠的脾脏，另一个进入淋巴结。评估了抗胸腺细胞血清对 MLR 和协同作用的影响。虽然存在微小差异并在本文中进行了描述，但我们的总体结果强烈表明，在我们的 MLR 协同实验中，我们可能正在处理与 Cantor 和 Asofsky 及其同事 (1,2,4,5,14) 描述的相同的 T1 和 T2 细胞亚群，在移植物抗宿主反应中表现出协同作用。
The present studies have shown that two subpopulations of thymus-dependent lymphocytes may act synergistically in the mixed lymphocyte reaction (MLR) in the mouse. One subpopulation was well represented in the young adult thymus and the other in lymph nodes. For optimum synergy, both populations must be allogeneic to the stimulator cells. Pretreatment of either population with mitomycin-C abolished synergy. Anti-θ serum abolished both MLR responding and synergizing activities of lymphoid cells. The two thymus-dependent subpopulations were both present in the spleen, and displayed different migratory patterns when injected into irradiated mice: one population went to spleens of the irradiated mice, the other to lymph nodes. The effects of anti-thymocyte serum on the MLR and upon synergy were assessed. While minor differences exist and are herein described, our overall results strongly suggest that in our experiments with synergy in MLR, we may be dealing with the same T1- and T2-cell subpopulations described by Cantor and Asofsky and coworkers (1, 2, 4, 5, 14) as displaying synergy in the graft-vs.-host reaction.