Forskolin cooperating with growth factor on generation of dopaminergic neurons from human fetal mesencephalic neural progenitor cells
Forskolin cooperating with growth factor on generation of dopaminergic neurons from human fetal mesencephalic neural progenitor cells
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DOI：
10.1016/j.neulet.2004.03.007
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发表时间：
2004-05-20
影响因子：
2.5
通讯作者：
Wang, XM
中科院分区：
文献类型：
--
作者：
Wang, X;Li, XX;Wang, XM
测试了毛喉素的共活化能力，以增强成纤维细胞生长因子（FGF）8对人胎儿中脑神经祖细胞（NPC）的多巴胺能（DA能）分化的功能。当单独用FGF 8处理NPC时，DA能表型轻度表达。加入10 μ M毛喉素增加DA能神经元的数量，与50 ng/ml FGF 8协同作用。这些细胞产生神经递质DA，这是通过高效液相色谱法测量。逆转录-聚合酶链反应（RT-PCR）分析表明，分化后的细胞表达DA能神经元发育相关基因酪氨酸羟化酶（TH）、核受体相关因子1（Nurr 1）和D2受体（D2 R），表明在本实验条件下可以获得成熟的DA能神经元。结果表明，毛喉素加FGF 8可能有助于更有效地生产DA能神经元从人类来源的NPC治疗神经退行性疾病。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Forskolin was tested for its co-activating ability to enhance the function of fibroblast growth factor (FGF) 8 on dopaminergic (DAergic) differentiation from human fetal mesencephalic neural progenitor cells (NPCs). When NPCs were treated with FGF8 alone, the DAergic phenotype was expressed lightly. The addition of 10 muM forskolin increased the number of DAergic neurons, cooperating with 50 ng/ml FGF8. These cells produced neurotransinitter DA, which was measured by high-performance liquid chromatography. Reverse transcriptase-polymerase chain reaction analysis demonstrated that differentiated cells expressed DAergic development-relative genes tyrosine hydroxylase (TH), nuclear receptor-related factor 1 (Nurr1) and D2 receptor (D2R), indicating that matured DAergic neurons could be obtained under these present conditions. The results suggest that forskolin plus FGF8 may contribute to more efficient production of DAergic neurons from human-derived NPCs for therapy of neurodegenerative, diseases. (C) 2004 Elsevier Ireland Ltd. All rights reserved.