Cyclic Stretch Augments Production of Neutrophil Chemokines, Matrix Metalloproteinases, and Activin A in Human Endometrial Stromal Cells.
Cyclic Stretch Augments Production of Neutrophil Chemokines, Matrix Metalloproteinases, and Activin A in Human Endometrial Stromal Cells.
复制标题
循环拉伸增强人子宫内膜基质细胞中中性粒细胞趋化因子、基质金属蛋白酶和激活素 A 的产生。
DOI：
10.1111/aji.12359
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发表时间：
2015
影响因子：
3.6
通讯作者：
Osuga Y.
中科院分区：
文献类型：
--
作者：
Izumi G;Koga K;Takamura M;Osuga Y.
问题：本研究的目的是验证我们的假设：月经期间发生在子宫的收缩活动诱导了增强子宫内膜重塑的生化因子。研究方法：采用Flexercell Tension系统对人子宫内膜基质细胞（ESC）进行循环拉伸，模拟月经期间的收缩活动。采用elisa和特异性测定法测定CXCL8、CXCL1、MMPs和激活素A的浓度和活性。用迁移试验评估中性粒细胞趋化活性。结果循环拉伸显著诱导ESC细胞分泌CXCL8、CXCL1及中性粒细胞趋化。拉伸也增加了MMP‐1、MMP‐2和MMP‐3活性、激活素A分泌和ESC活性。结论月经期间子宫收缩活动通过诱导趋化因子分泌、MMP表达、活性和中性粒细胞趋化性参与子宫内膜重塑。
ProblemThe aim of this study was to test our hypothesis: Contractile activity that occurs in the uterus during menstruation induces biochemical factors that enhance remodeling of the endometrium.Method of StudyCyclic stretch, mimicking contractile activity during menstruation, was applied to human endometrial stromal cells (ESC) using the Flexercell Tension system. The concentration and activity of CXCL8, CXCL1, MMPs, and activin A were measured using ELISAs and specific assays. Neutrophil chemotactic activity was evaluated using migration assays.ResultsCyclic stretch significantly induced ESC secretion of CXCL8 and CXCL1 and neutrophil chemotaxis. Stretch also increased MMP‐1, MMP‐2, and MMP‐3 activity, activin A secretion, and activity in ESC.ConclusionThese results indicate that the contractile activities of the uterus during menstruation contribute to the remodeling of the endometrium, by inducing chemokine secretion, MMP expression, activity, and neutrophil chemotaxis.