SPATULA, a gene that controls development of carpel margin tissues in Arabidopsis, encodes a bHLH protein.
SPATULA, a gene that controls development of carpel margin tissues in Arabidopsis, encodes a bHLH protein.
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发表时间：
2001-04
期刊：
影响因子：
4.6
通讯作者：
M. Heisler;A. Atkinson;Yasmin H. Bylstra;Rebecca L. Walsh;D. Smyth
中科院分区：
文献类型：
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作者：
M. Heisler;A. Atkinson;Yasmin H. Bylstra;Rebecca L. Walsh;D. Smyth
涉及该基因突变体的研究表明，它促进了心皮边缘和由心皮边缘衍生的花粉道组织的生长。我们发现它编码碱性-螺旋-环-螺旋转录因子家族的一个新成员。在边缘和花粉道组织的整个发育过程中，抹刀都有表达，证实了它在调节它们生长方面的作用。它也在许多其他组织中表达，在这些组织中，它可能冗余地控制生长，包括茎顶端分生组织的外围区，以及叶、花瓣、雄蕊和根中的特定组织。在气孔、瓣索和瓣裂区的表达表明，在脱落过程中还有一个额外的作用。尽管它在第一轮器官中的表达被A功能基因APETALA2抑制，但它的表达不需要其他心皮发育基因螃蟹CLAW和AGAMOUS的功能。此外，我们已经证明，在Ettin突变体中看到的对雌雄异株模式形成的干扰在很大程度上可以归因于异位铲子的作用。Ettin的作用似乎是负向调节发育中的雌蕊远轴区的刮片表达。Spatula是植物中第一个在花器官发生中发挥作用的基本螺旋环螺旋基因。
Studies involving mutants of the gene SPATULA indicate that it promotes the growth of carpel margins and of pollen tract tissues derived from them. We show that it encodes a new member of the basic-helix-loop-helix family of transcription factors. SPATULA is expressed in marginal and pollen tract tissues throughout their development confirming its role in regulating their growth. It is also expressed in many other tissues where it may act redundantly to control growth, including the peripheral zone of the shoot apical meristem, and specific tissues within leaves, petals, stamens and roots. Expression in the stomium, funiculus and valve dehiscence zone indicates an additional role in abscission. SPATULA expression does not require the function of the other carpel development genes CRABS CLAW and AGAMOUS, although its expression is repressed in first whorl organs by the A function gene APETALA2. Further, we have shown that disruptions to gynoecial pattern formation seen in ettin mutants can largely be attributed to ectopic SPATULA action. ETTIN's role seems to be to negatively regulate SPATULA expression in abaxial regions of the developing gynoecium. SPATULA is the first basic-helix-loop-helix gene in plants known to play a role in floral organogenesis.