Regenerative properties of fetal sheep tendon are not adversely affected by transplantation into an adult environment
Regenerative properties of fetal sheep tendon are not adversely affected by transplantation into an adult environment
复制标题
DOI：
10.1002/jor.20271
复制
发表时间：
2006-11-01
影响因子：
2.8
通讯作者：
Soslowsky, Louis J.
中科院分区：
文献类型：
--
作者：
Favata, Michele;Beredjiklian, Pedro K.;Soslowsky, Louis J.
每年，肌腱损伤导致大量的肌肉骨骼疾病。虽然新的手术技术和康复方案改善了临床结果，但术后疤痕仍然是肌腱修复中最成问题的方面。与这种典型的纤维化模式相反，最近的研究表明胎儿肌腱能够愈合且不留疤痕。然而，这种再生愈合模式是胎儿组织本身固有的还是其环境的结果尚不清楚。因此，本研究的目的是检查成人环境对成人和胎儿肌腱愈合的影响。我们假设，移植到成人环境中的受伤胎儿肌腱组织在受伤后会保留再生愈合模式，表现出正常的组织学和机械特性。我们的结果支持这一假设。组织学分析显示，成人肌腱移植物在受伤后发生了相当大的变化，而胎儿移植物则没有表现出异常。受伤的成人肌腱在伤口部位也表现出 TGF-β1、bFGF 和 CD44 水平升高，而胎儿样本在损伤反应中几乎没有或没有显示出这种变化。我们的生物力学研究数据进一步证实了这些观察结果，与未受伤的成人肌腱相比，受伤的成人肌腱的刚度、模量和几乎所有粘弹性特性均显着下降，而胎儿样本显示受伤组和未受伤组之间的机械特性没有差异。因此，我们的调查结果表明，成人环境并不是无疤痕修复的障碍，并且这种能力是胎儿肌腱本身固有的。我们的研究还开始深入了解控制这种再生反应的机制。 (c) 2006 年骨科研究学会。
Tendon injuries account for a significant number of musculoskeletal afflictions each year. While new surgical techniques and rehabilitation protocols have led to improved clinical outcomes, postsurgical scarring remains the most problematic aspect of tendon repair. In contrast to this typical pattern of fibrosis, recent studies have shown that fetal tendon is capable of healing without scar. However, whether this regenerative healing pattern is intrinsic to the fetal tissue itself or the result of its environment is not known. Thus, the objective of this study is to examine the influence of an adult environment on healing in adult and fetal tendons. We hypothesized that injured fetal tendon tissue transplanted into an adult environment would retain a regenerative healing pattern after injury, demonstrating normal histological and mechanical properties. Our results support this hypothesis. Histological analyses revealed considerable alterations in adult tendon transplants after injury while fetal transplants showed no abnormalities. The injured adult tendons also demonstrated elevated levels of TGF-beta l, bFGF, and CD44 at the wound site, whereas the fetal specimens showed little or no such changes in response to injury. The data from our biomechanical studies further corroborate these observations, with significant decreases in the stiffness, modulus, and almost all viscoelastic properties in wounded versus unwounded adult tendons, and fetal specimens showing no differences in mechanical properties between the wounded and unwounded groups. Thus, the results of our investigation demonstrate that the adult environment is not an impediment to scarless repair and that this capability is intrinsic to the fetal tendon itself. Our study also begins to provide insight into the mechanisms controlling this regenerative response. (c) 2006 Orthopaedic Research Society.