P2X7 gene polymorphisms and risk assessment for pulmonary tuberculosis in Asian Indians.
P2X7 gene polymorphisms and risk assessment for pulmonary tuberculosis in Asian Indians.
复制标题
DOI：
10.3233/dma-2010-0682
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Hasan Q
中科院分区：
文献类型：
--
作者：
Sambasivan V;Murthy KJ;Reddy R;Vijayalakshimi V;Hasan Q
目的：肺结核（PTB）是发病和死亡的主要原因。巨噬细胞在结核病的免疫发病机制中发挥重要作用。细胞外 ATP 通过激活嘌呤能 P2X7 受体诱导巨噬细胞杀菌活性。这项病例对照研究评估了 PTB 患者和健康对照中 -762 T/C、1513A/C 和 1729T/A P2X7 多态性的关联，以确定是否与罹患该疾病的风险存在关联。 材料和方法：使用 PCR 和 RFLP 分析对 256 名个体进行 P2X7 基因分型，其中包括 156 名活动性 PTB 患者和 100 名年龄和性别的匹配健康志愿者，无临床症状或 PTB 家族史作为对照。 结果：卡方检验显示 PTB 患者和对照者之间的 -762 C 等位基因存在显着差异； p=0.0051（OR 1.6972，CI 95% 1.1839 至 2.4332），1729 T 等位基因被发现与 PTB 呈正相关； p < 0.0005（OR-2.4623，CI 95% 1.6376 至 3.7022）。 1513A/C多态性在两组之间没有显示出任何显着差异。 意义：该研究揭示了 P2X7-762C 等位基因和 P2X7 1729T 等位基因受体多态性与亚裔印度人群中的 PTB 存在显着相关性。需要确定使用这些等位基因作为生物标志物来识别患有结核病高风险的个体。
Objective: Pulmonary tuberculosis (PTB) is a leading cause of morbidity and mortality. Macrophages play an important role in the immunopathogenesis of tuberculosis. Extracellular ATP induces macrophage bactericidal activity through activation of the purinergic P2X7 receptor. This case- control study assesses the association of −762 T/C, 1513A/C and 1729T/A P2X7 polymorphisms in patients with PTB and healthy controls to establish association if any with risk of developing the disease. Materials and methods: The genotyping for P2X7 was carried out using PCR and RFLP analysis in 256 individuals, which included 156 active PTB patients and 100 age and sex, matched healthy volunteers with no clinical symptoms or family history of PTB as controls. Results: A chi square test showed a significant difference between the PTB patient and controls for −762 C allele; p=0.0051 (OR 1.6972, CI 95% 1.1839 to 2.4332) and1729 T allele was found to be positively associated with the PTB; p < 0.0005 (OR-2.4623, CI 95% 1.6376 to 3.7022). 1513A/C polymorphism did not show any significant difference between the two groups. Significance: The study revealed a significant association of P2X7-762C allele and P2X7 1729T allele receptor polymorphisms with PTB in Asian Indian population. The use of these alleles as biomarkers for identifying individuals at high risk of developing TB needs to be ascertained.