Detection of alprazolam (Xanax) and its metabolites in urine using dual capillary column, dual nitrogen detector gas chromatography.
Detection of alprazolam (Xanax) and its metabolites in urine using dual capillary column, dual nitrogen detector gas chromatography.
复制标题
采用双毛细管柱、双氮检测器气相色谱法检测尿液中的阿普唑仑（Xanax）及其代谢物。
DOI：
10.1093/jat/11.6.247
复制
发表时间：
1987
影响因子：
2.5
通讯作者：
A. Joern
中科院分区：
文献类型：
--
作者：
W. A. Joern;A. Joern
阿普唑仑（Xanax）是一种相当新的，但非常受欢迎的苯二氮卓类镇静剂。1985年，它在全国急诊室药物中毒名单上排名第六。开发了一种程序，允许在尿液标本中检测阿普唑仑及其主要尿液代谢物，α -羟阿普唑仑和3-羟甲基-5-甲基三唑基氯苯甲酮。采用双毛细管柱、双氮气检测器气相色谱系统进行可靠鉴定。通过乙酰化代谢产物，提高了色谱性能。测定了方法的线性度、重现性、检出限和回收率等特征。该方法通过对服用阿普唑仑的住院患者的尿液标本进行检测。在大多数情况下，α -羟阿普唑仑的浓度最高，从内源性尿液物质中分离效果最好。进行了单剂量浓度-时间研究，测定了阿普唑仑和代谢物浓度在48小时内的时间过程。
Alprazolam (Xanax) is a fairly new, yet very popular benzodiazepine tranquilizer. In 1985, it was 6th on a nationwide list of drugs mentioned in emergency room drug poisonings. A procedure was developed that allows alprazolam and its main urinary metabolites, alpha-hydroxyalprazolam and 3-hydroxymethyl-5-methyltriazolyl chlorobenzophenone, to be detected in urine specimens. A dual capillary column, dual nitrogen detector gas chromatographic system was used for reliable identification. Improved chromatographic performance was obtained by acetylating the metabolites. Method characteristics such as linearity, reproducibility, limit of detection, and recovery were determined. The method was tested by assaying urine specimens from hospitalized patients who had been ingesting alprazolam. In most cases, alpha-hydroxyalprazolam was found in the highest concentration and separated best from endogenous urine substances. A single dose concentration-time study was performed, and the time course of alprazolam and metabolite concentrations over 48 hours was determined.