PHOSPHOINOSITIDES .3. ENZYMIC HYDROLYSIS OF INOSITOL-CONTAINING PHOSPHOLIPIDS
PHOSPHOINOSITIDES .3. ENZYMIC HYDROLYSIS OF INOSITOL-CONTAINING PHOSPHOLIPIDS
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DOI：
10.1042/bj0790193
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发表时间：
1961-01-01
影响因子：
4.1
通讯作者：
HAWTHORNE, JN
中科院分区：
文献类型：
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作者：
KEMP, P;HUBSCHER, G;HAWTHORNE, JN
一种水解磷酸肌醇的可溶性酶已从大鼠肝脏中纯化了50倍。它水解脑中的磷脂酰肌醇和二磷酸肌醇部分，但不攻击磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酸或心磷脂。为了获得最佳活性，需要Ca2+离子。描述了该制剂的两种最佳pH值和其它性质。发现在制备物中存在两种独立的水解作用，导致从磷酸肌醇形成肌醇单磷酸（磷酸肌醇酶C）和甘油磷酸肌醇（磷酸肌醇酶A和B）。
A soluble enzyme which hydrolyses phosphoinositides has been purified 50-fold from rat liver. It hydrolyzes phosphatidyl-inositol and the diphosphoinositide fraction from brain, but does not attack phosphatidylcholine, phosphatidylethanolamine, phosphatidyl-serine, phosphatidic acid or cardiolipin. For optimum activity Ca2+ ions are required. Two pH optima and other properties of the preparation are described. Two independent hydrolytic actions were found to be present in the preparation leading to the formation from phos -phatidylinositol of inositol monophosphate (phosphoinositidase C) and of glycerylphosphorylinositol (phosphoinositidases A and B.).