Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range
Far-field fluorescence microscopy with three-dimensional resolution in the 100-nm range
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DOI：
10.1046/j.1365-2818.1997.2410797.x
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发表时间：
1997-07-01
影响因子：
2
通讯作者：
VanderVoort, HTM
中科院分区：
文献类型：
--
作者：
Hell, SW;Schrader, M;VanderVoort, HTM
我们报告三维（3D）显微镜与近各向同性的分辨率在λ/5-λ/10范围内。我们的方法结合了4Pi共焦双光子荧光显微镜与图像恢复。用密集聚集的珠子以及小鼠成纤维细胞中的F-肌动蛋白纤维证明了3D分辨率。与未恢复的双光子共焦图像的比较揭示了一个总的不确定性体积减少到15倍。
We report three-dimensional (3D) microscopy with nearly isotropic resolution in the lambda/5-lambda/10 range. Our approach combines 4Pi-confocal two-photon fluorescence microscopy with image restoration. The 3D resolution is demonstrated with densely clustered beads as well as with F-actin fibers in mouse fibroblast cells. A comparison with unrestored two-photon confocal images reveals a total reduction of the uncertainty volume up to a factor of 15.