INDUCED SYNTHESIS OF ENZYMES IN BACTERIA ANALYZED AT THE CELLULAR LEVEL
INDUCED SYNTHESIS OF ENZYMES IN BACTERIA ANALYZED AT THE CELLULAR LEVEL
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DOI：
10.1016/0006-3002(53)90057-2
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发表时间：
1953-01-01
期刊：
影响因子：
--
通讯作者：
BENZER, S
中科院分区：
文献类型：
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作者：
BENZER, S
噬菌体被用作鉴别装置，用于确定细菌（大肠杆菌B）培养细胞中诱导酶（β - d -半乳糖苷酶）的分布。结果表明，酶的细胞分布由酶释放量与细胞裂解量的曲线形状决定。这种分布是研究在培养诱导到不同水平的特定酶活性。在适当的诱导条件下，β -半乳糖苷酶的诱导合成在所有细胞中以统一的速率进行。在其他条件下，可能会出现高度的异质性。当含酶细胞在没有诱导剂的情况下繁殖时，最初存在的酶在子细胞中均匀地分裂。描述了用于确定细菌培养细胞中诱导酶的分布的另一种方法。该方法依赖于这样一个事实，即某些噬菌体在细胞内繁殖过程中对紫外光失活的抵抗力会发生渐进式变化。在适当的条件下，斑块形成能力的存活作为辐射剂量的函数可以指示一种因子的分布，如必需的诱导酶。
Bacteriophage is utilized as a discriminating device for determining the distribution of an inducible enzyme (beta-D-galactosidase) in the cells of a culture of bacteria (Escherichia coli B). It is shown that the cellular distribution of the enzyme is given by the shape of the curve of enzyme released vs. number of cells lysed. This distribution is investigated in cultures induced to various levels of specific enzyme activity. Under suitable conditions of induction, the induced synthesis of beta-galactosidase proceeds at a uniform rate in all the cells. Under other conditions a high degree of heterogeneity may occur. When enzyme-containing cells multiply in the absence of inducer, the initially-present enzyme is uniformly partitioned among the daughter cells. Another method is described for determining the distribution of an inducible enzyme in the cells of a bacterial culture. The method depends on the fact that certain phages undergo progressive changes in resistance to UV light inactivation during their intracellular reproduction. Under the proper conditions survival of plaque-forming ability as a function of radiation dose can indicate the distribution of a factor such as an essential inducible enzyme.