Comparison of water- and alkali-extracted polysaccharides from Fuzhuan brick tea and their immunomodulatory effects in vitro and in vivo.
Comparison of water- and alkali-extracted polysaccharides from Fuzhuan brick tea and their immunomodulatory effects in vitro and in vivo.
复制标题
茯砖茶水提、碱提多糖及其体内外免疫调节作用比较
DOI：
10.1039/d1fo02944d
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发表时间：
2022
期刊：
影响因子：
6.1
通讯作者：
Cao Yungang
中科院分区：
文献类型：
--
作者：
Sun Yujiao;Wang Fan;Liu Yang;An Yuye;Chang Dawei;Wang Jiankang;Xia Fei;Liu Ning;Chen Xuefeng;Cao Yungang
比较水提法和碱辅助提取法对茯砖茶多糖结构特征和免疫调节活性的影响。结果表明，水提和碱提FBTP分子量相近，但单糖组成不同，其中碱提FBTP的糖醛酸基组成与半乳糖醛酸（GalA）相同，且产量较高。体外实验表明，A-FBTP对巨噬细胞吞噬能力、酸性磷酸酶活性和一氧化氮（NO）分泌有较强的促进作用。在体内研究中，A-FBTP通过增强环磷酰胺（CTX）诱导的免疫抑制小鼠的身体特征、抗氧化活性、免疫应答和肠粘膜屏障，显示出有希望的调节免疫失衡的作用。此外，A-FBTP补充有效地改善了CTX诱导的肠道微生物群生态失调，包括促进有益细菌（例如，乳酸杆菌）和短链脂肪酸（SCFA）产生细菌（例如，毛螺菌科（Lachnospiraceae）、普雷沃菌科（Prevotellaceae）和瘤胃球菌科（Ruminococcaceae）），沿着减少潜在致病微生物（例如，脱硫弧菌科（Desulfovibrionaceae）和螺杆菌（Helicobacter））。这些结果表明，碱法提取法是一种从茯砖茶中提取高质量酸性多糖的有效方法，A-FBTP可作为新型益生元和免疫调节剂应用于食品中。
In the present study, the purpose is to compare the effect of water extraction and alkali-assisted extraction on the structural characteristics and immunomodulatory activity of polysaccharides from Fuzhuan brick tea (FBTPs). The results indicated that water-extracted FBTPs (W-FBTPs) and alkali-extracted FBTPs (A-FBTPs) had similar molecular weights but different monosaccharide compositions, of which A-FBTPs had a higher yield and uronic acid groups corresponding to galacturonic acid (GalA). Moreover, A-FBTPs had stronger ability to promote phagocytic capacity, acid phosphatase activity and nitric oxide (NO) secretion in macrophages in vitro. In the in vivo study, A-FBTPs exhibited a promising effect to adjust the immune imbalance by enhancing the body features, antioxidant activities, immune response and intestinal mucosal barrier in cytoxan (CTX)-induced immunosuppressive mice. Besides, A-FBTP supplementation effectively improved CTX-induced gut microbiota dysbiosis, including promoting the abundance of beneficial bacteria (e.g., Lactobacillus) and short chain fatty acid (SCFA)-producing bacteria (e.g., Lachnospiraceae, Prevotellaceae and Ruminococcaceae), along with reducing the growth of potentially pathogenic microbes (e.g., Desulfovibrionaceae and Helicobacter). These findings suggested that alkaline extraction might be a promising way to obtain high-quality acidic polysaccharides from Fuzhuan brick tea (FBT), and A-FBTPs could be developed as novel potential prebiotics and immunomodulators for further application in food formulations.