Acid-responsive composite hydrogel platform with space-controllable stiffness and calcium supply for enhanced bone regeneration
Acid-responsive composite hydrogel platform with space-controllable stiffness and calcium supply for enhanced bone regeneration
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DOI：
10.1016/j.cej.2020.125353
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发表时间：
2020-09-15
影响因子：
15.1
通讯作者：
Wu, Jun
中科院分区：
文献类型：
--
作者：
Bao, Ziting;Gu, Zhipeng;Wu, Jun
许多用于骨再生的水凝胶支架由于机械性能和钙供应不可控以及缺乏突出的成骨功能而不能满足临床需要。在此，开发了一种新型的酸响应性复合水凝胶支架平台，以提供可控的刚度和钙供应，用于增强骨再生。为此，选择Pluronic F127二丙烯酸酯（F127-DA）作为模型支架基质材料，因为其具有良好的生物相容性和易于配制的能力。选择纳米碳酸钙作为复合材料，以提供酸响应特性和受控的钙供应。酸响应性纳米CaCO 3可以导致纳米CaCO 3在酸处理下在水凝胶中的空间控制分布，从而有效调节水凝胶支架的机械性能。此外，该水凝胶可以有效地调节Col 1、BMP 2和OPN的表达，这有利于成骨。在兔颅骨骨缺损模型中的体内研究表明，这种F127-DA/纳米CaCO 3复合水凝胶平台比纯F127-DA水凝胶在骨再生方面表现更好。因此，这种响应性复合水凝胶平台为开发用于有效骨修复的先进复合水凝胶支架提供了简单而稳健的策略。
Many hydrogel scaffolds for bone regeneration do not satisfy clinical needs due to uncontrollable mechanical properties and calcium supply and a lack of outstanding osteogenic functions. Herein, a novel acid-responsive composite hydrogel scaffold platform was developed to provide controllable stiffness and calcium supply for enhanced bone regeneration. For this purpose, pluronic F127 diacrylate (F127-DA) was selected as the model scaffold matrix material due to its good biocompatibility and easy formulation capability. Nano-CaCO3 was chosen as the composite material to provide an acid-responsive property and controlled calcium supply. The acid-responsive nano-CaCO3 may lead to space-controlled distribution of nano-CaCO3 in a hydrogel under acid treatment, leading to effective regulation of the mechanical properties of the hydrogel scaffold. Furthermore, the hydrogel may efficiently modulate the expression of Col1, BMP2 and OPN, which are beneficial for osteogenesis. In vivo studies in a rabbit skull bone defect model indicated that this F127-DA/nano-CaCO3 composite hydrogel platform performed better in bone regeneration than a pure F127-DA hydrogel. Therefore, this responsive composite hydrogel platform provides a simple and robust strategy for developing advanced composite hydrogel scaffolds for effective bone repair.