Transcription-repair coupling factor is involved in carbon catabolite repression of the Bacillus subtilis hut and gnt operons.
Transcription-repair coupling factor is involved in carbon catabolite repression of the Bacillus subtilis hut and gnt operons.
复制标题
转录修复耦合因子参与枯草芽孢杆菌 hut 和 gnt 操纵子的碳分解代谢物抑制。
DOI：
10.1046/j.1365-2958.1998.00751.x
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发表时间：
1998
影响因子：
3.6
通讯作者：
Fisher,SH
中科院分区：
文献类型：
--
作者：
Zalieckas,JM;WrayJr,LV;Ferson,AE;Fisher,SH
ABacillus subtilismutant that partially relieves carbon catabolite repression (CCR) of thehutoperon was isolated by transposon mutagenesis. Characterization of this mutant revealed that the transposon had inserted into the gene,mfd, that encodes transcription–repair coupling factor. The Mfd protein is known to promote strand‐specific DNA repair by displacing RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the DNA damage site. A set of transcriptionallacZfusions was used to demonstrate that themfdmutation relieves CCR ofhutandgntexpression at thecis‐actingcresequences located downstream of the transcriptional start site but does not affect CCR at sites located at the promoters. CCR of theamyEandbglPHgenes, which containcresequences that overlap their promoters, is not altered by themfdmutation. These results support a model in which the Mfd protein displaces RNA polymerase stalled at downstreamcresites that function as transcriptional roadblocks and reveal a new role for Mfd in cellular physiology.
