Marine chronology based on 14C dating on diatoms proteins
Marine chronology based on 14C dating on diatoms proteins
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DOI：
10.1016/j.marchem.2007.12.008
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发表时间：
2008-02
期刊：
影响因子：
3
通讯作者：
C. Hatté;G. Hodgins;A. Jull;Bongi Bishop;B. Tesson
中科院分区：
文献类型：
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作者：
C. Hatté;G. Hodgins;A. Jull;Bongi Bishop;B. Tesson
我们提出了一种新的方法来获得硅藻晶体（silafin）固有蛋白质的放射性碳年代。通过确定缺乏碳酸钙的沉积物岩心样品的年龄模型，该方法将改进对基于硅藻的海洋或湖泊古代用物的解释。为了准备用加速器质谱法进行放射性碳测年，从岩心沉积物样品中浓缩硅藻。通过化学和物理处理，硅藻体从任何表面结合的有机物中解脱出来，这将在本文后面讨论。用HF溶解法从硅藻晶体的蛋白石基质中释放出其固有的化合物。该方法不同于利用制备色谱法从复杂的有机混合物中提取特定化合物的方法，因为我们消除了大部分受污染的有机物质。该方法的优点是不需要大量的成本投资，并且可以添加到制备色谱方案中，以确保测年的可靠性。这种方法应用于从南大洋收集的海洋岩心样本，该样本跨越了最后一个气候周期。我们报告了在化学处理的每个步骤中获得的有机物质的放射性碳定年结果，从散装到塞拉芬及其解释。
We present a new method of obtaining radiocarbon dates for proteins intrinsic to diatom frustules (sillafin). By asserting age models for sediment core samples that lack calcium carbonate, this method will improve interpretations of diatom-based paleoproxies for either marine or lacustrine. In preparation for radiocarbon dating by Accelerator Mass Spectrometry, diatoms were concentrated from core sediment samples. The diatoms frustules were freed of any surface-bound organic matter, through chemical and physical treatments that will be discussed later in this paper. Compounds intrinsic to diatoms frustules were released from their opal matrix by HF dissolution. This method differs from approaches based on specific compound extractions from complex organic mixture by preparative chromatography because we eliminated most of the contaminated organic matter. The advantage of our method is that it does not require heavy cost investment and it can be added to a preparative chromatography protocol to ensure dating reliability. This method was applied to samples from a marine core collected in the Southern Ocean, that spans the last climatic cycle. We report on the radiocarbon dating results obtained on organic matter at each step of the chemical treatment, from bulk to sillafin and their interpretation.